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Background and purpose: Melatonin is a neurohormone with important physiologic and pharmacologic role in human body especially in circadian rhythm .In recent years, some progress has been achieved to show its role in regulating in prevention of cancer especially breast and colon cancer. According to this background and as there was not any precise cellular research about the role of melatonin in gastric cancer in which this study this study has been aimed. Materials and methods: In this study, we used MTT assay procedure. Also, we have provided AGS and MKN-45 cell line from national cell bank, Institute Pasteur of Iran. The cells were cultured in RPMI medium in 5% CO2, 370C in 96 wells culture plate and then were incubated with melatonin and cisplatin (as positive control) for 48 hr. in 5 different concentrations. The proliferation index as cell viability was achieved and compared with controls groups with ELISA concerning Formazan crystal color absorbance between 450-690 nm. Results: Our results showed that melatonin in 12.5-200 µM has significant anti-proliferative effects in AGS cells and in 50-200 µM in MKN-45 compared with control and these results were in parallel with the effects of cisplatin. Conclusion: According to our data, we have shown that melatonin in a dose -dependent manner has antiproliferative effect in gastric adenocarcinoma cells and this effect in AGS cells was more potent than MKN-45 but more studies are needed to find the kind of receptors and the interacellular signaling pathways.

Keywords: Melatonin, gastric adenocarcinoma, Proliferation, MTT assay, Cisplatin

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