Zarsyfi1 M, Hoseeini Kahnouei M A, Rezazadeh H, Mahmoudi M H, Purrashidy Basharabadi A, Alahtavakoli M. Protective Effect of Combination Therapy of Local Brain Hypothermia and Decompressive Craniectomy on Stroke Model in Rat. J Mazandaran Univ Med Sci 2014; 24 (113) :81-90
URL:
http://jmums.mazums.ac.ir/article-1-3810-en.html
Abstract: (7579 Views)
Background and purpose: Both hypothermia and Decompressive craniectomy (DC) have been
shown to reduce ischemic injury in experimental middle cerebral artery occlusion (MCAO). This study
was designed to evaluate the effect of delayed combination of DC and Local Hypothermia (LH) on infarct
size, neurological outcome and blood brain barrier (BBB) disruption in a rat model of stroke.
Material and Methods: MCAO was induced in 36 Wistar rats assigned to three groups of
control, Decompressive Craniectomy (DC) and combination of Hypothermia and Craniectomy (HC).
Infarct size and BBB disruption were measured 48 hour after ischemia insult. Neurological deficits were
assessed at 24 and 48 hours after stroke using sticky tape test, hanging-wire test and Bederson’s scoring
system. BBB disruption was measured by Evans blue dye leakage.
Results: Compared to the control group, infarct size reduced significantly in DC and HC groups
(P<0.001), however, combination therapy was more neuroprotective compared to craniectomy alone
(P<0.01). Compared to the control group, BBB disruption was significantly reduced in DC (P<0.05) and
HC (P<0.01).While compared to the control group sticky tape test (P<0.05 at 24 h P<0.001 at 48 h) and
hanging-wire test (P<0.05) showed better behavioral performance only in HC, and Bederson test showed
improved behavioral functions of HC animals (P<0.01). At the same time neurological deficits also
decreased in HC group (P<0.05 at 24 h P<0.01 at 48 h) compared to the DC group.
Conclusion: According to this study although both delayed local hypothermia and craniectomy
are protective after stoke, combination therapy of them is more neuroprotective than given alone.