Volume 25, Issue 123 (4-2015)                   J Mazandaran Univ Med Sci 2015, 25(123): 32-44 | Back to browse issues page

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Sobhani M, Rouzbehi A, Mahmoodi R, Sobhani Z. The Protective Effect of Melatonin on Sperm Morphology and Histology of the Rat Testis Damage Induced by Cadmium Chloride. J Mazandaran Univ Med Sci. 2015; 25 (123) :32-44
URL: http://jmums.mazums.ac.ir/article-1-5535-en.html
Abstract:   (4332 Views)
Background and purpose: Oxidative stress inhibits sperm motility and changes in shape and be individuals leads to sterility. Cadmium, increased free radical production in cells and cell tissue damage. The best way to deal with it, increasing antioxidants secret among the antioxidants melatonin as an antioxidant was studied. Materials and methods: 64 male Wistar rats over 6-8 weeks were divided into 8 groups of 8, (two control groups and six experimental groups). The first group was injected intra peritoneal with a single daily dose of 10 mg / kg melatonin injection for 30 days, the second group injected intra peritoneal with a single daily dose of 15 mg / kg melatonin injection for 30 days, Group III intra peritoneal single daily dose of 20 mg / kg melatonin injection for 30 days. Group IV as a positive control group was injected intra peritoneal with a single daily dose of 2 mg / kg for 30 days Cadmium chloride injection, the fifth group intra peritoneal with a daily single dose of 2 mg / kg - Cadmium chloride with 10 mg daily of g / kg melatonin injection for 30 days , six daily intra peritoneal with a single dose of 2 mg / kg daily- Cadmium chloride with a single 15 mg / kg melatonin injection for 30 days , Group VII intra peritoneal dose single daily dose of 2 mg / kg daily Cadmium chloride with a single 20 mg / kg melatonin were injected for 30 days .Group of Eight negative control group received intra peritoneal injection of physiological solution. Testicular volume and weight measurements and cell counts based on morphology and testis were examined. Results: Results showed a significant reduction of the layer thickness and the number of germ cells Cadmium chloride group (P <0/05) and this was significantly different between the groups receiving melatonin to increase the layer thickness and the number of germ cells (P <0/05) compared to the control group and the group receiving both drugs simultaneously, particularly the significant differences in the groups receiving 20 mg of melatonin was compared to the group receiving cadmium alone (P <0/05). Conclusion: The present study shows the effects of cadmium chloride damage sperm activity and morphology of testis and melatonin are the property antioxidant, causing the low the effects of cadmium chloride on testicular improve spermatogenesis, increasing the number of cells and germinal layer thickness and ultimately will improve fertility.
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Type of Study: Research(Original) | Subject: anatomy

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