Volume 25, Issue 128 (9-2015)                   J Mazandaran Univ Med Sci 2015, 25(128): 97-107 | Back to browse issues page

XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Mahdiyoun S M, Ahanjan M, Goudarzi M, Rezaee R. Prevalence of Antibiotic Resistance in Methicillin-resistant Staphylococcus aureus and Determining Aminoglycoside Resistance Gene by PCR in Sari and Tehran Hospitals . J Mazandaran Univ Med Sci. 2015; 25 (128) :97-107
URL: http://jmums.mazums.ac.ir/article-1-6140-en.html
Abstract:   (5285 Views)

Abstract Background and purpose: Staphylococcus aureus is one of the major causes of community and hospital-acquired infections. Aminoglycosides are potent bactericidal agents that are often used in combination with beta-lactam or glycopeptide in treatment of staphylococcal infections. The aim of this study was to investigate the prevalence of the aminoglycoside modifying enzyme aac(6΄)-Ie/aph(2”) genes and mecA. Materials and methods: In current study, 174 clinical isolates of MRSA from different clinical specimens were tested for antibiotic susceptibility using the Kirby Bauer method. MRSA isolates were detected by disc diffusion method using 1 ug oxacillin and 30 ug cefoxitin discs. Then, MRSA strains were further analyzed for detection of mecA and aminoglycoside resistant aac (6΄)-Ie/aph (2˝) genes by PCR. Results: All strains were resistant to oxacillin and cefoxitin. The highest and lowest resistances were found against erythromycin (85.1%) and trimethoprim/sulfamethoxazole (24.7%), respectively. In PCR, mecA gene was detected in 100% of the strains and 77% of the strains were found to harbor aac(6′)/aph(2//)-Ia gene. Conclusion: According to this study, the prevalence of aminoglycosides resistant genes is increasing in MRSA isolates.

Full-Text [PDF 708 kb]   (2370 Downloads)    
Type of Study: Research(Original) | Subject: psychology

Add your comments about this article : Your username or Email:
CAPTCHA code

Send email to the article author


© 2018 All Rights Reserved | Journal of Mazandaran University of Medical Sciences

Designed & Developed by : Yektaweb