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Background and purpose: Escherichia coli is the most prevalent etiologic agent of urinary tract infection. Enzymatic inactivation of aminoglycosides by aminoglycoside-modifying enzymes is the main mechanism of resistance to these antibiotics in E. coli. The aim of this study was detecting the ant(2′′)-Ia gene among aminoglycoside resistant clinical isolates of E. coli using PCR method. Materials and methods: 276 clinical isolates of E. coli were collected and their antibiotic susceptibility patterns were determined by disk diffusion method for gentamicin, amikacin, tobramycin, kanamycin and netilmicin paper disks considering CLSI principles. Chromosomal DNA of the isolates was also extracted using DNA extraction kits and PCR method was used to detect the ant (2′′)-Ia gene Results: Results of disk diffusion showed that 24.63%, 23.18%, 21.01%, 6.15% and 3.62% of E. coli isolates were resistant to tobramycin, kanamycin, gentamicin, netilmicin and amikacin, respectively. ant (2′′)-Ia gene was found in 47.88% of E. coli isolates Conclusion: Tracing the transfer routs among different bacteria very important as there is a high prevalence of resistance toward aminoglycoside antibiotics due to its transfer among bacteria by transferable elements such as transposons and plasmids.

Keywords: Aminoglycoside Resistance, Escherichia coli, ant(2′′)-Ia gene, Urinary tract infection

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