Background and purpose: Osteoporosis is a common disease characterized by low bone mass that can harm health. Lactoferrin increase osteogenesis. PerFlourooctyl Bromide is a neutral particle that used as a drug carier.

Materials and methods: In this study, lactoferrin (LF) of Camel milk were isolated. PFOB-NEP was prepared using an oil-in-water emulsion method (O/W). and then evaluated by The zeta potential measurement methods and tryptophan fluorescence spectroscopy before and after loaded by lactoferrin. The concentrations of 25, 50 and 100 µg/mL of  LF were loaded on perflourooctylbromide -NEPs. The effects of standard LF, the LF, and LF loaded on PFOB-NEPs on growth of osteoblasts Cell Line (MG-63) was studied using MTT assay.

Results: The results showed that the LF and the LF loaded on PFOB-NEPs significantly increased Osteoblast cells proliferation. But there was no significant difference between the LF and LF uploaded on PFOB-NEPs (α<1%). But there were significant differences of growth in different concentrations of lactoferrin relative to each other in both lactoferrin and lactoferrin loaded on nanoparticles (α<5%).

Conclusion: The results proved that LF of camel milk causes the growth of osteoblasts cells in MG-63 cell lines. It is also possible to load LF on PFOB-NEPs and this nanoparticle could be used as carriers of lactoferrin.