Showing 2 results for Mondanizadeh
Malihe Bagheri, Ghasem Mosayebi, Mahdieh Mondanizadeh,
Volume 30, Issue 192 (1-2021)
Abstract
Background and purpose: T-cell acute lymphoblastic leukemia (T-ALL) is a type of blood malignancy caused by changes in the precursors of T lymphocyte cells. The PTEN gene is one of the most common tumor suppressor genes that mutates in most human cancers, including T-ALL. Therefore, it is important to identify miRNAs that target the PTEN gene in T-ALL. For this purpose, in the present study, miRNAs targeting PTEN gene in T-ALL patients were examined.
Materials and methods: The miRNAs targeting PTEN was predicted and selected using bioinformatics. A total of 40 plasma samples were collected from T-ALL patients and healthy individuals. After RNA extraction and synthesis of cDNA, the expression levels of predicted miRNA and PTEN were assessed by Real time PCR in REST 2009. Statistical analysis was performed in SPSS V16 and P<0.05 were considered significant.
Results: Bioinformatics showed that miR-1297 target the PTEN gene. Compared to healthy individuals, in T-ALL patients, the expression levels of miR-1297 and PTEN gene in the plasma significantly increased (45.93, P= 0.001) and decreased (0.191, P= 0.001), respectively.
Conclusion: The present study showed that miR-1297 is likely to target the PTEN gene. Therefore, miR-1297 could serve as a new and non-invasive biomarker in diagnosis and treatment
of T-ALL.
Maryam Zand, Mahdieh Mondanizadeh,
Volume 31, Issue 206 (3-2022)
Abstract
Cell Free miRNAs are small, non-coding molecules that can be secreted into the bloodstream in very stable forms. These types of miRNAs, like intracellular miRNAs, participate in the control of many biological processes and are expressed in both natural and pathological conditions. Quantitative and qualitative changes in expression of circulating miRNAs are associated with the onset and progression of cancer, but can be easily detected by some molecular biology methods. The most common methods for diagnosis, prognosis, and treatment of most cancers are aggressive, so finding accurate and non-invasive methods for early diagnosis, monitoring and control of cancer are essential. The circulating miRNAs affect not only cells near the site of secretion but also distant tissues, so, they can lead to cancer progression. Herein, we examined studies based on the identification of circulating miRNAs as non-invasive diagnostic biomarkers, prognostic, and of course important for the treatment of various types of cancers.
