Showing 2 results for Tahmasebi Fard
Seyedeh Maryam Seyed Ali Roteh, Zahra Tahmasebi Fard, Afshin Abdirad, Zahra Moeini,
Volume 22, Issue 87 (Apr 2012)
Abstract
Background and purpose: Ovarian tumor is a common neoplasm of the female genital tract and one of the most lethal gynecologic malignancies. The etiology of ovarian cancer remains unclear. Oncogenic viruses can contribute in different stages of the carcinogenic process. Papilloma viruses are Oncogenic viruses that could induce proliferation of epithelial cells.
Materials and methods: In this study samples were 50 Paraffin-embedded blocks of ovarian carcinoma tissue and 58 normal Paraffin-embedded blocks (without malignancy) as control group. After DNA extraction, all samples were analyzed to detect beta-globin gene and suitable samples were screened for presence of L1 HPV-Common. The data was analyzed using one-way ANOVA in SPSS 15.
Results: All positive samples for beta-globin gene were amplified by HPV-common primers. Among the samples seven of 44 (15.90%) ovarian carcinoma and five of 50 (10%) normal ovarian tissue were found positive for the common marker of HPV. Overall, 12 of 94 samples were positive (12.76%). The correlation between HPV infection and ovarian cancer was not statistically significant (p= 0.397).
Conclusion: No significant correlation was found between HPV infection and ovarian cancer. Therefore, the results of this study do not confirm the role of HPV in ovarian cancer.
Zahra Tahmasebi Fard, Farzaneh Tafvizi, Leyla Khojareh,
Volume 22, Issue 95 (11-2012)
Abstract
Background and purpose: Breast cancer is one of the most common malignancies in women and early diagnosis of this cancer is a key element for successful treatment. Breast cancer is a multistep disease in which a virus can play a role. Epstein-Barr Virus (EBV) is identified as an important factor in human cancer. This study investigated the relationship between EBV and breast cancer.
Materials and methods: Sixty seven paraffin-embedded tissue blocks of breast cancer were used and 53 paraffin blocks of non-cancerous breast tissue (26 fibroadenoma and 27 fibrocystic) were collected as the controls. DNA was extracted using the Roche kit, then all samples were analyzed for the presence of beta-globin gene and suitable samples were evaluated for the existence of DNA-EBV through Polymerase Chain Reaction (PCR). X2 test of homogeneity and SPSS ver.15 were used to analyze the data.
Results: Two cases negative for beta-globin gene in paraffin-embedded tissue blocks of breast cancer were excluded from the study. EBV was detected by PCR in 23 (35.38%) cases of breast cancer specimens and in 11 (20.75%) cases among the control samples (7 fibroadenoma and 4 fibrocystic). From the total of 118 samples 34 cases (81.28%) were found positive.
Conclusion: This study found significant correlation between EBV infection and breast cancer. Further epidemiological, biological and molecular assessments are needed to confirm the possible association between this virus and the process of carcinogenesis.
