Showing 5 results for Yazdian-robati
Rezvan Yazdian-Robati, Zeinab Asghari Ayask, Sepideh Arabzadeh, Maryam Hashemi, Fatemeh Kalalinia,
Volume 33, Issue 2 (12-2023)
Abstract
Background and purpose: The osteogenic differentiation of MSCs plays a key role in bone tissue engineering and regenerative medicine, making it essential to explore natural compounds that may enhance this process. This aim this study was to investigate the osteogenic potential of both aqueous and alcoholic extracts derived from the root of Rubia tinctorum on rat bone marrow-derived mesenchymal stem cells (BM-MSCs).
Materials and methods: Aqueous and alcoholic extracts were prepared from the root of Rubia tinctorum. Rat BM-MSCs were isolated and cultured and their osteogenic differentiation was assessed in the presence of varying concentrations of the extracts using alizarin red staining and alkaline phosphatase (ALP) activity. Cytotoxic effect of root extract of Rubia tinctorum was evaluated using MTT assay.
Results: The MTT assay demonstrated that the root extract of Rubia tinctorum was cytotoxic at 1000 µg/ml. Additionally, the alizarin red assay showed that the most significant color intensity at 1 and 10 µg/ml concentrations on the 14th day. In terms of bone differentiation induction which measured via the alkaline phosphatase test, both the aqueous and alcoholic extracts at 1 and 10 µg/ml concentrations led to substantial increases (14.9- and 14.57-fold for the aqueous extract and approximately 7.37- and 7-fold for the alcoholic extract, respectively) in alkaline phosphatase activity compared to the negative control.
Conclusion: This study sheds light on the osteogenic effects of Rubia tinctorum root extracts, emphasizing their potential as natural agents in promoting bone regeneration and tissue engineering applications. Further investigations into the underlying molecular mechanisms and in vivo studies are warranted to comprehensively evaluate the feasibility of utilizing these extracts for therapeutic bone regeneration purposes
Nafiseh Jirofti, Azadeh Shahroodi, Jebreil Movaffagh, Bibi Sedigheh Fazly Bazzaz, Rezvan Yazdian-Robati, Maryam Hashemi,
Volume 33, Issue 220 (5-2023)
Abstract
Background and purpose: Topical antibiotic medication is an alternative method in treatment of local infections, especially osteomyelitis. Currently several biomaterials are used for this purpose. The present study focused on the fabrication and characterization of chitosan and Vancomycin (VCM)-loaded chitosan (CS)-hydroxyapatite (HA)-gelatin (G) bead in treatment of osteomyelitis.
Materials and methods: CS/G/HA/VCM scaffold was prepared by cross-linking using ionotropic gelling. The morphological and mechanical properties, loading efficiency and antibacterial properties were investigated and toxicity assay of fabricated structure was also analyzed.
Results: The lyophilization reduced the area, environment, and sphericity of beads. CS/G/HA/VCM structure showed smaller area (4.5 mm2) and perimeter (180 µm) compared to other structures. Suitable morphology and porosity in fabricated beads were confirmed by SEM images in CS/G/HA and CS/G structures. Reduction of strength was observed after adding VCM into fabricated structure, but this happened less in CS/G/HA/VCM structure. Release on day 14 was 58% for CS/G/HA/VCM structure. AddingVCM into CS/VCM (1.88 Mpa) and CS/G/VCM (10.46 Mpa) structures decreased the mechanical strength while it increased the mechanical strength in CS/G/HA/VCM (83.21 Mpa) structure. No toxic activity of fabricated structures was seen against NIH 3T3 cells. The results of the antimicrobial activity against Staphylococcus aureus showed that chitosan beads containing VCM (CS/VCM, CS/G/VCM, CS/G/HA/VCM) could significantly inhibit the growth of S. aureus compared to the positive control (VCM 5%)(P<0.001).
onclusion: According to current study, the CS/G/HA/VCM structure can be introduced as a promising candidate in treatment of osteomyelitis.
Reza Iraei, Parisa Khanicheragh, Hadis Musavi, Mohammad Yazdi, Negin Chavoshinejad, Rezvan Yazdian-Robati,
Volume 33, Issue 229 (1-2024)
Abstract
Lipid accumulation in the liver is associated with non-alcoholic fatty liver disease (NAFLD), with obesity and insulin resistance being the main contributing factors. The AMPK/SIRT1 signaling pathway plays a crucial role in addressing lipid metabolism issues. Recent studies have demonstrated that the AMPK/SIRT1 signaling axis is involved in preventing and reducing liver damage. Upregulation of AMPK/SIRT1 can regulate lipid metabolism and oxidation in liver cells. In NAFLD, increased activity of AMPK/SIRT1 can inhibit the synthesis of fatty acids and cholesterol by down-regulating adipogenesis genes (FAS, SREBP-1c, ACC, and HMGCR). Therefore, activation of the AMPK/SIRT1 signaling pathway represents a potential therapeutic target for liver disorders. This review summarizes the most recent studies on the AMPK/SIRT1 pathway signaling axis and the mechanisms of herbal activators of the AMPK/SIRT1 pathway in non-alcoholic fatty liver
Tahereh Molania, Parastoo Namdar, Atieh Taheri, Melika Mollaei, Maedeh Salehi, Rezvan Yazdian-Robati,
Volume 34, Issue 232 (4-2024)
Abstract
Background and purpose: Due to the absence of a conclusive cure for a severely painful mouth ulcer, the creation of a medication to regulate this ailment is greatly advantageous. Folinic acid is a derivative of 5-formyl tetrahydrofolic acid. Unlike folic acid (the synthetic form of folate), folinic acid is a form of folate found naturally in foods. Folinic acid can be converted to other active forms of folate in the body and has the activity of the complete vitamin folic acid. Since folinic acid has wound-healing effects, it may play an important role in accelerating the healing of aphthous wounds. By determining the optimal dose, folinic acid can be suggested as a recommended treatment option for people with oral ulcers such as aphthous wounds. The purpose of this study is to investigate the effect of folinic acid on the growth of gingival fibroblast cells as a treatment for mouth ulcers.
Materials and methods: During this experimental study, human gingival fibroblast cell lines were cultured in sterile conditions in a DMEM culture medium of 10% bovine serum and 1% penicillin and tetracycline antibiotics at 37 degrees. These cells were exposed to different concentrations of folinic acid drug (5, 20, 25, 30, 40, 50, 80, 100 μM). The MTT method was used to evaluate cell viability and determine IC50 (inhibitory concentration). In this experiment, due to the uncertainty of its range of toxicity on cells, the relative toxicity was determined in a pilot phase and with few repetitions. Each concentration was repeated four times and incubated at different times (24, 48, and 72 hours). After the incubation time, the supernatant of each well was discarded and 100 μl of MTT solution was added to each well. After four hours of incubation, the supernatant was discarded and 100 μL of DMSO was added. Then, using an ELISA reader, the optical absorbance of each well was measured at a wavelength of 540-690 nm. Finally, IC50, which indicates the drug concentration necessary to inhibit 50% of cell growth, was calculated using the growth curve, and the results were analyzed using SPSS software version 19.
Results: In this study, the effect of folinic acid cytotoxicity on human gingival fibroblast cell line (HGF1) in a cell culture medium was investigated three times in different concentrations. The results showed that 70% of the cells were still alive in 24 hours up to a concentration of 100 μM, which can indicate the effective use of this drug for the treatment of pest damage. In 48 hours, IC50= 1.78 μM was obtained, which indicates that in studies with a time limit of 48 hours, up to a dose of 80 μM of folinic acid can be used to use the therapeutic effects of this drug. In 72 hours, IC50 was calculated as 66.7 μM.
Conclusion: These findings provide valuable information about the dose-response relationship and the impact of folic acid on HGF1 cells. It indicates that higher concentrations of folic acid are needed initially to achieve a significant reduction in cell growth, but with longer exposure, lower concentrations can be effective.
Fatemeh Rezaie, Majid Saeedi, Rezvan Yazdian-Robati, Javad Akhtari,
Volume 34, Issue 234 (6-2024)
Abstract
Functioning as the human body's most extensive organ, the skin exhibits an intricate, stratified architecture. This architecture consists of distinct layers: the epidermis, dermis, and hypodermis. Collectively, these layers play a crucial role in safeguarding the body and upholding its general well-being. The aging process of the skin is complex and is influenced by a wide range of internal and external factors. The loss of skin elasticity and firmness is a consequence of these factors, which ultimately contribute to signs of aging such as wrinkles, skin discoloration, and diminished radiance as people get older. A comprehensive understanding of these factors and their impact on the skin can lead to the development of more effective methods and products for skin care, capable of slowing down this process and improving skin quality. Recent advances in nanotechnology, driven by increasing consumer demand for beauty products, have prompted the development of novel methods of skincare that are aimed at minimizing the appearance of symptoms of aging. Therefore, nanotechnology in skin care has emerged as a recognized novel and effective approach that can provide efficient solutions for combating skin aging. Nano lipid carriers have been introduced as one of the key innovations in this field. These carriers can effectively enhance the penetration of active cosmetic and skincare ingredients through the branched layers of the epidermis, which serve as the main barrier to the penetration of substances into the skin. These nanocarriers enhance the stability of active ingredients present in cosmetic and skincare formulations. When it comes to cosmetics and skincare products, nano lipid carriers have several distinctive qualities that add to their potential to improve efficacy while simultaneously minimizing side effects. They facilitate controlled release and long-term diffusion of active ingredients, ensuring continuous and uniform distribution of active compounds in the skin. Hence, they improve the biocompatibility of ingredients, thereby reducing skin irritation and enhancing skin tolerance to active substances. A wide range of nanocarriers is utilized in the dermal delivery of cosmetic and skincare products. These include nanoemulsions, liposomes, lipid nanoparticles, ethosomes, niosomes, transferosomes, nanocrystals, as well as polymeric and mineral nanocarriers. Each of these nanocarriers exhibits specific properties and mechanisms of action that effectively improve the penetration and efficacy of active ingredients into the deeper layers of the skin. In the present article, various types of nano lipid carriers have been examined as effective vehicles for delivering active compounds into the skin layers, along with their mechanisms of action. Furthermore, the latest anti-aging and anti-wrinkle products containing these nanocarriers have been discussed. Scientific investigations have demonstrated that the use of nano lipid carriers in the formulation of anti-aging products can significantly improve skin rejuvenation and regeneration. These products, by enhancing the permeability and stability of active compounds, exhibit better efficacy in reducing wrinkles and fine lines, increasing skin firmness and elasticity. It is also anticipated that with further advancements in this field, more innovative products will be introduced to the market to meet diverse consumer needs.