Zahra Bakhtiari, Rasool Shahrooz, Abas Ahmadi, Farhad Soltanali,
Volume 24, Issue 118 (11-2014)
Abstract
Background and purpose: Oxidative stress induced by cyclophosphamide (CP), a chemotherapeutic agent, causes vulnerability of sperm and declining the fertility power. Then in this study the effect of crocin (saffron extract) on amelioration of these side effects was investigated.
Materials and methods: In this study 24 adult male mice were divided in three groups (n=8). Control group received normal saline (0.2 ml/day, IP), CP group received cyclophosphamide
(15 mg/kg/week, IP), and CP+Cr group received crocin (200 mg/kg/day, IP) along with CP for 35 days. Then the animals were euthanized and malondialdehyde (MDA) in testis was assayed. After collection of sperm from caudate of epididymis, the rate of DNA damage was calculated by acridine orange staining. After stimulation of ovulation in 72 female adult mice, oocytes were collected and transferred in HTF medium that contained BSA. Then, in-vitro fertilization (IVF) was done with capacitated sperms. The rate of fertilization and primitive embryonic growth were evaluated for 120 hours.
Results: The results showed increase in fertilization, two cell embryos and blastocysts in CP+Cr group compared to those of the CP group (P<0.05). Also, the crocin in CP+Cr group prevented the increase of total number of arrested embryos and percentage of arrested embryos type I, II, and III (P<0.05). Decrease in MDA assay and sperms with damaged DNA were observed more in CP+Cr group (P<0.05).
Conclusion: This study showed the efficiency of crocin in amelioration of fertility and growth of primitive embryo in animals that received CP.
Aliakbar Mozaffari, Rassul Shahrooz , Abbas Ahmadi, Hassan Maleki Nejad, Karim Mardani,
Volume 27, Issue 155 (12-2017)
Abstract
Background and purpose: Oxidative stress is amongst the factors that change the parameters of sperm and embryo development. This study investigated the protective effects of vitamin E on sperm parameters and In Vitro Fertilization (IVF).
Materials and methods: In the present study, male mice were divided into four groups to recieve physiological saline (control group), PHZ, vitamin E +PHZ, and vitamin E. Female mice were used for In Vitro Fertilization. Thirty five days after treatments, the male mice were euthanized and their sperm parameters were studied. Stimulating ovule development was done. The animals were euthanized, and then the ova were fertilized. Data analysis was conducted in SPSS applying ANOVA.
Results: Compared to the control group, the mean percentage of sperms with damaged DNA, lack of nuclear maturation, and abnormal morphology increased significantly in PHZ group, which was improved by antioxidants (P<0.05). In PHZ group, the mean percentage of fertility, blastocyst, and blocked embryos increased significantly compared to the control group (P<0.05). But considerable improvements were seen in these parameters after receiving vitamin E.
Conclusion: Current study showed that vitamin E could improve sperm indicators and embryological development in vitro. Therefore, it is recommended in order to improve the aforementioned parameters.
