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Showing 3 results for Interleukin-6
Effects of Quercetin on Signaling Proteins (pSTAT3, pERK1/2, pAKT) and Interleukin-6 Gene Expression in Prostate Cancer PC3 cells
Esfandiar Heidarian, Sayed Asadollah Amini, Akram Abbasi-Veldani, Keihan Ghatreh-Samani,
Volume 26, Issue 144 (1-2017)
Abstract

Background and purpose: Interleukin-6 (IL-6) causes the progression of prostate cancer through pSTAT3, pERK1/2, and pAKT cell signaling proteins. Quercetin, an herbal antioxidant, has anti-tumor effect. The aim of this study was to evaluate the effects of quercetin on IL-6 gene expression, and the above cellular signaling proteins in PC3 prostate cancer cells.

Materials and methods: In this experimental study, PC3 cells were treated with different concentrations of quercetin at 0, 10, 50, and 100 μM.  Then, IL-6 concentration was determined in cell culture media. Also, total RNA and the cellular signaling proteins aforementioned were extracted from PC3 and used for determining IL-6 gene expression by quantitative real-time RT-PCR and western blot analysis, respectively.

Results: The quercetin IC50 for PC3 prostate cancer cells was 100 μM. Elevation of quercetin concentration in cell culture media increased the IL-6 gene expression and protein synthesis. At 50 and 100 μM of quercetin, IL-6 protein synthesis increased significantly (P<0.05) to 13.36% and 36.86%, respectively, compared to those in control. Furthermore, quercetin suppressed pSTAT3, pERK1/2, and pAKT cell signaling proteins at dose concentrations more than 150 μM.

Conclusion: The effects of quercetin on PC3 cells could have resulted from reduction of pSTAT3, pERK1/2, pAKT, induction of the oxidative stress and generation of reactive oxygen species. Therefore, quercetin can be considered as a useful therapeutic agent in treatment of prostate cancer.


Comparative Study on the Role of Serum Levels of Interleukin-6 and Interleukin-8 in Predicting the Severity of COVID-19 and Prognosis in Patients Admitted to Ghaemshahr Razi Hospital
Alireza Davoudi, Sahar Azadeh, Narges Najafi, Bahareh Soleimanpourian, Abbas Alishahi, Armaghan Mokhtariani, Mazaher Azordeh, Reyhaneh Shojaei-Asrami, Mohammad Khademloo, Leila Delavarian, Fatemeh Ahangarkani,
Volume 32, Issue 217 (1-2023)
Abstract
 Background and purpose: The Coronavirus Disease 2019 (COVID-19) pandemic, caused by severe respiratory syndrome coronavirus 2 (SARS-CoV-2) resulted in a worldwide emergency. Various studies show conflicting and diverse results on the role of inflammatory cytokines in predicting the severity and prognosis in patients with COVID-19. The aim of this study was to compare the serum levels of interleukin-6 (IL-6) and interleukin-8 (IL-8) in predicting the severity of COVID-19 and prognosis in patients admitted to Qaemshahr Razi Hospital.
Materials and methods: In this cross-sectional prospective study, the serum levels of interleukin 8 and 6 were measured in 51 patients with COVID-19 and compared with 25 healthy individuals in Qaemshahr Razi Hospital, 2021. ELISA method was used to measure these cytokines and data analysis was performed in SPSS V25.
Results: The serum levels of IL6 and IL8 in the patient group were about 4 times and 6 times higher than those of the control group, respectively. Average levels of IL-6 (P=0.004) and IL-8 (P<0.001) were significantly higher in patients with severe COVID-19 compared to moderate form and control group. There was no correlation between the duration of hospitalization and the level of IL-6 (P=0.1), while there was a significant correlation between the length of hospital stay and the level of IL-8 (P=0.012).
Conclusion: IL-6 and IL-8 serum levels in patients with COVID-19 can be helpful in predicting the severity of disease and prognosis of patients.

 
Evaluation of the effect of different pulp capping materials on the secretion of Interleukin-6 from human dental pulp stem cells: an in vitro study
Naghmeh Ahmadiankia, Anahita Lotfizadeh, Mehdi Bagheri, Mozhgan Fazli, Salma Omidi,
Volume 34, Issue 236 (8-2024)
Abstract
Background and purpose: Regenerative treatments in endodontics are biological treatments to manage immature teeth, remove inflamed and necrotic tissue, and create a suitable environment for tissue repair. In these treatments, substances with reparative properties of dental pulp and dentin are placed on the pulpal tissue. Interleukin-6 is one of the cytokines secreted in response to various immune activities of the body and studies have demonstrated its vital role in mineralization induction and hard tissue formation. Recently, calcium silicate-based materials have been widely implemented in dentistry due to their great biocompatibility and hard tissue formation induction. Considering the role of IL-6 in the regulation of stem cells, the purpose of this study is to compare the effect of CEM cement, Biodentine, TheraCal LC, and MTA on the level of IL-6 secretion by dental pulp stem cells.
Materials and methods: In this laboratory study, 4 types of cement were used as the main groups, and 1 control group contained culture medium without cement. Discs were prepared under sterile conditions in six-well plates with a diameter of 35 mm and a height of 2 mm and they were given time to set for 48 hours. Human pulp stem cells were obtained from Iran's Center for Biological Resources and cultured in a culture medium containing DMEM with 15% FBS and 1% penicillin-streptomycin (10,000 units/ml) as antibiotics. Pulp stem cells at passages 3-5 were used for experiments. Discs of each cement were prepared under sterile conditions and according to the manufacturer's instructions. To prepare the extract of each cement, the DMEM cell culture medium was placed near these discs for 24 hours. Then, human dental pulp stem cells (hDPSCs) were cultured in the vicinity of the disc extract for 72 hours. After that, this extract was collected and the level of IL-6 secretion in it was measured by the ELISA method. The experiments were repeated 3 times. Then the obtained data were entered into SPSS 16 software and analyzed using analysis of variance and the Kruskal-Wallis test.
Results: The results showed that the highest level of IL-6 secretion by cells was in the presence of TheraCal LC, Biodentine, and CEM, respectively; But there was no significant difference between the groups. The average level of IL-6 secretion in the presence of MTA was lower than in other groups and this difference was significant (P<0.01).
Conclusion: The results of the present study showed a lower level of IL-6 cytokine secretion by pulpal stem cells in the presence of MTA compared to TheraCal, CEM, and Biodentine types of cement. Considering the role that IL-6 can have in osteogenic and odontogenic activities, the results of this study can be useful in establishing information in the field of introducing the most suitable cements for clinical procedures.
 
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