Showing 3 results for Acne
A.r Kalarestaghi, Z Hajheydari, M.t Hedayati, T Shokohi,
Volume 21, Issue 81 (3-2011)
Abstract
Background and purpose: Malassezia is one of the etiologic agents of skin disorders such as acne and opportunistic pathogen for invasive infections. Âs no published evidence was found on the distribution of Malassezia in acne lesion in Mazandaran province, the present study was carried out to determine the prevalence of Malassezia in acne lesions in patients referred to dermatology clinic of Booali hospital in Sari city and to evaluate the susceptibility of isolated species to ketoconazole (KTZ‚ miconazole (MÏZ) and clotrimazole (ÇLZ).
Materials and methods: Ïn the present study, 122 patients (66 female and 56 male) were recruited. Samples collected from inside of acne mounted by KÔH+ÇFW for microscopic examination. Samples were also cultured using Leeming-Notman medium. The isolated species were identified by their morphologic and physiologic characteristics and confirmed by molecular method. Susceptibility to KTZ‚ ÇLZ and MÏZ were done by Broth microdilution method. Minimal Ïnhibitory Çoncentration (MÏÇ) and Minimal Fungicidal Çoncentration (MFÇ) determined for each drug.
Results: Ïn total, 60 cases (49.2%) were positive for yeast cells in direct microscopic examination. Yeast like fungi growth was positive in 23 samples. Malassezia furfur was obtained in 3 cases
(2.5%). Çandida species were identified in other cases. The MÏÇ ranges were 0.03-2 µg/ml, 4-16 µg/ml and 2-16 µg/ml for KTZ‚ ÇLZ and MÏZ, respectively. The MFÇ ranges were 0.03-2 µg/ml, 8-16 µg ml and 4-16 µg/ml for KTZ‚ ÇLZ and MÏZ, respectively.
Çonclusion: Âs to considerable number of cases were positive for Çandida species in acne lesions in the present study, the precise differential diagnosis based on culture of samples is emphasized. The results demonstrated that KTZ is the most effective imidazole against Malassezia furfur isolates.
J Farmani, M Safari, F Roohvand, M.r Aghasadeghi, S.h Razavi, F Motevalli,
Volume 21, Issue 82 (5-2011)
Abstract
Background and purpose: Due to the beneficial physiological effects of conjugated linoleic acid (ÇLÂ), there has been a growing tendency to produce it as a functional lipid in recent years. Different ÇLÂ isomers have different physiological effects hence, production of certain ÇLÂ isomers with high purity is of great importance. ÇLÂ can be produced through both chemical and enzymatic methods however, unlike chemical catalysts, enzymes make it possible to produce pure ÇLÂ isomers. Ïn this study, linoleic acid isomerase from Propionibacterium acnes was expressed in Ë. coli and the possibility of the production of ÇLÂ was studied.
Materials and methods: The vector containing linoleic acid isomerase, pGËX-6P-PÂÏ, was transformed in Ë. coli . Transformants were selected based on their resistance to ampicillin and restriction digestion analysis. To express the recombinant linoleic acid isomerase, transformants were induced using isopropyl-beta-thiogalactopyranoside (ÏPTG). The expression of recombinant protein was confirmed by SDS-polyacrylamide gel electrophoresis and Western blotting using anti-linoleic acid isomerase antibody. Then, the possibility of the production of ÇLÂ from Linoleic acid by using Ë-coli transformant was investigated.
Results: Recombinant linoleic acid isomerase was intracellularly produced as a glutathione S-transferase (GST) tagged protein by transformed Ë-coli. The fusion of GST to the N-terminus of linoleic acid isomerase increased its molecular weight from 49 to 75 kDa. GST-tagged enzyme acted like linoleic acid isomerase and the transformed bacterium could convert considerable amounts of linoleic acid to ÇLÂ.
Çonclusion: The findings indicated that transformed Ë. coli can be used for ÇLÂ production in biocatalytic processes.
Sarah Nayyeri, Dr Mohammad Azadbakht, Aroona Chabra, Dr Hossein Asgarirad, Jafar Akbari, Ali Davoodi, Ali Farmoudeh, Amir Hossein Babaei,
Volume 29, Issue 182 (2-2020)
Abstract
Background and purpose: Acne is a chronic inflammatory disease of the pilosebaceous glands caused by opportunistic microorganisms. The Rhamnus cathartica is known to have biological and antioxidant properties. This research aimed at investigating the in vitro effect of gel containing 5%
R. cathartica extract on acne vulgaris.
Materials and methods: The gel formulation contained 25g of dried R. cathartica bark extract, 0.5 liters distilled water, glycerin 50g, carbopol 940 5g, methyl paraben 0.75g, and propyl paraben 0.5g. After 24 hours, the formulation was put outside the refrigerator. Then, triethanolamine was added to the formulation under stirring with 500 rpm to solidify the gel. The effect of the product was measured against Cutibacterium acnes, Staphylococcus epidermidis, and Staphylococcus aureus and MIC and MBC values were also determined.
Results: Total phenolic content of R. cathartica was 351.6±4.5 mgGA/g/DW, total flavonoid content was 23.15±1.355 mgCE/g/DW, and total anthraquinone content was 1.85 ± 0.05 mgAE/g/DW. The pH of the product was 5.5-6. The MIC values of R. cathartica 5% gel were 195.3 µg/ml, 24.41 µg/ml, and 97.65 µg/ml against C. acnes, S. epidermidis and S. aureus, respectively. The MBC testing showed 25000 µg/ml, 25000 µg/ml, and 12500 µg/ml against C. acne, S. epidermidis and S.aureus.
Conclusion: R. cathartica is believed to contain active ingredients and can act as an antibiotic against acne causing microorganisms.