Showing 6 results for Mast Cell
Hamideh Kadeh, Shirin Saravani,
Volume 26, Issue 142 (11-2016)
Abstract
Background and purpose: Mast cells as the multi-functional and complex cells play a key role in tumor progression. Due to the higher malignant potential of Oral Squamous cell carcinoma (OSCC) compared with cutaneous squamous cell carcinoma (cSCC), the purpose of this study was to compare the mast cells count in squamous cell carcinoma of the skin and oral cavity and evaluate their correlation with the degree of differentiation of these tumors.
Materials and methods: In this retrospective study, 30 cases of OSCC and 30 cases of cSCC were investigated. Toluidine blue staining was used to identify the number of mast cells in samples. Data was analyzed in SPSS (V.21) applying Mann-Whitney, Kruskal Wallis and Pearson correlation coefficient tests.
Results: The mean count of mast cells in OSCC and cSCC were 46±29.68 and 63.66±40.85, respectively, indicating a significant difference (P=0.012). The mean counts of mast cells in grade I OSCC and grade III of cSCC were higher than those of other grades but the differences were not statistically significant (P>0.05).
Conclusion: The average number of mast cells in oral squamous cell carcinoma was less than that in cutaneous squamous cell carcinoma. It seems that other factors such as cytokines and chemokines released from tumor cells and inflammatory cells other than mast cells are effective in the progression of oral squamous cell carcinoma.
Maryam Seyyed Majidi, Farhad Dabbagh Sattari, Roghaieh Faeli Ghadikolaei, Hemmat Gholinia,
Volume 26, Issue 144 (1-2017)
Abstract
Background and purpose: Periodontitis and gingivitis are inflammatory diseases in supporting tissues of tooth. Mast cells are inflammatory cells that can participate in destruction of connective tissue in periodontal diseases. In this study we evaluated mast cells count in gingival tissues in individuals with periodontal diseases and compared that with those in healthy people.
Materials and methods: This case-control study was done in 60 samples obtained from patients with advanced periodontitis (n=15), moderate periodontitis (n=15), gingivitis (n=15), and healthy tissue samples (n=15, control group). Biopsy specimens obtained during flap and crown lengthening surgery were stained with toluidine blue and hematoxylin eosin.
Results: Mast cells count were significantly different between four groups (P<0.001). In fact a significant difference was found in healthy status compared to that in moderate periodontitis, advanced periodontitis and gingivitis (P<0.001) but it did not show any significant difference in advanced and moderate periodontitis compared with that in gingivitis (P>0.05). Also, mast cells count did not reveal any significant difference between advanced periodontitis and moderate periodontitis (P>0.05). Moreover, we found no significant relationship between mast cells count and degree of microscopic inflammation (P=0.09)
Conclusion: Mast cells are believed to play a role in pathogenesis of periodontal diseases, but further studies are suggested to clarify this role.
Najmeh Jafari, Seyed Hossein Tabatabaei, Forough Salehi,
Volume 29, Issue 175 (8-2019)
Abstract
Background and purpose: Inflammatory cells including mast cells, with the production of inflammatory mediators such as heparin and histamine participate in the process of inflammation and jaw resorption. Considering the increasing growth of cysts and the role of mast cells in the process of bone resorption, the role of mast cells might be considered in pathogenesis and growth of cysts. The aim of this study was to evaluate the presence of mast cells in two most common odontogenic jaw cysts.
Materials and methods: Tissue sections of 20 cases of radicular cyst and odontogenic keratocyst (OKC) were stained with Giemsa and observed under optical microscope in five randomized areas of the subepithelial and deep zones. Data analysis was done in SPSS 23 applying ANOVA test, multiple comparison test, and t-test.
Results: The average number of mast cells in radicular cysts was higher than that in OKC. There was no significant difference in subepithelial zone of these cysts (P =0.093), while a significant difference was observed in deep zone of wall of radicular cysts and OKC (P =0.006).
Conclusion: Mast cells may play a role in the pathogenesis of radicular cysts, but, they may not be directly involved in the growth and pathogenesis of OKC.
Mehdi Mehdinezhad Roshan, Farshad Moharrami Kasmaie, Mohammad Kazemi Ashtiani, Mohammad Amin Abdollahifar, Saeideh Erfanian, Ibrahim Zarkesh, Davood Nasiry, Abbas Piryaei,
Volume 33, Issue 1 (11-2023)
Abstract
Background and purpose: One of the most complications of diabetes are chronic skin wounds. Recently Human Amniotic Membrane Scaffold (HAMS) used as a wound dressing. In this study, the impact of HAMS on the rate of wound closure and the number of mast cells in the granulation tissue of diabetic rats was evaluated.
Materials and methods: 45 diabetic rats were divided into 3 groups: healthy wound, diabetic wound, and diabetic wound transplanted with HAMS. 30 days after diabetes induction via injecting 55mg/kg of Streptozotocin, a circular wound with a diameter of 15mm was created. HAMS was transplanted to the wound and the percentage of wound closure and the density of type 1, 2 and 3 mast cells in the granulation tissue were evaluated on days 7, 14 and 21.
Results: HAMS transplantation significantly enhanced the wound closure compared to diabetic group (P<0.05). Comparing to healthy group, the number of all types of mast cells and their total number in diabetic group was significantly decreased on day 7 (P<0.001) and significantly increased on days 14 and 21 (P<0.001). However, these changes modulated in HAMS transplanted group, in which in all cases except for type 3 mast cell on day 7 there were significant differences compared to diabetic group (P<0.01). Moreover, in HAMS transplant group, the number of type 1 and 3 mast cells on day 14 reached the level of healthy group.
Conclusion: These results suggested that HAMS improves the diabetic wound healing via modulating inflammatory reactions by affecting the mast cells density in the granulation tissue.
Hamideh Kadeh, Shirin Saravani, Mahsa Mohammadpour,
Volume 33, Issue 223 (8-2023)
Abstract
Background and purpose: The definitive role of mast cells and angiogenesis in the pathogenesis of Peripheral giant cell granuloma (PGCG) of the oral cavity is not yet fully understood. The aim of this study was to evaluate the mast cell counts (MCs) and microvascular density (MVD) and investigating the relationship between them and PGCG of the oral cavity.
Materials and methods: In this descriptive-analytic study, 50 samples were selected from the archieve of oral pathology department in dental school at Zahedan University of Medical Sciences using census. The samples included 40 PGCG and 10 normal oral tissues that were stained by immunohistochemistry (CD31 antibody) and toluidine blue to identify MVD and MCs, respectively. Data were analyzed using T-test, Pearson correlation test in SPSS V.21.
Results: The mean number of mast cells was 38.51±36.43 in PGCG and 14±3.21 in normal tissues indicating significant difference between the two groups (P= 0.041). Findings showed a significant difference in MVD between PGCG group (93.34±64.43) and normal tissues (41.9±16.7) (P=0.017). MVD and MCs below the epithelium were found to be higher compared with those in deeper area of connective tissue. No correlation was found between the MCs and MVD (r=0.003, P=0.982).
Conclusion: In the present study, mast cells count and microvascular density were higher in PGCG than normal tissue which could explain the role of mast cells and the subsequent process of angiogenesis in the pathogenesis of this reactive lesion of the oral cavity, but no correlation was found between the mast cells count and microvascular density.
Najmeh Jafari, Seyed Mostafa Mahmoudi, Fatemeh Taherneghad,
Volume 34, Issue 242 (2-2025)
Abstract
Background and purpose: Oral squamous cell carcinoma is the most common malignancy of the oral cavity. In solid tumors, cancer cells and stromal cells (fibroblasts, inflammatory cells, endothelial cells) play a role in tumor progression, angiogenesis, local invasion, and metastasis. Mast cells contribute to carcinogenesis by releasing chemical mediators in their granules through various pathways, including suppressing the immune system, enhancing angiogenesis, destroying the extracellular matrix, and increasing the mitosis of tumoral cells. Several studies have investigated the role of mast cells in oral squamous cell carcinoma. While some suggest that mast cells play an angiogenic and tumorigenic role, others do not confirm this, highlighting the contradictory findings in this area. In this study, we discuss the role of mast cells in oral squamous cell carcinoma.
Materials and methods: The mean number of mast cells was significantly lower in higher microscopic grades compared to poor grades (P<0.05). However, there was no significant difference in the average number of mast cells based on age, gender, or location of occurrence (P>0.05).
Results: the mean number of mast cells was significantly lower in microscopic grade and higher than poor (P-value˂0/05). But there was no significant difference in the average number of mast cells based on age, gender and place of occurrence (P˃0/05).
Conclusion: The significant increase in the average number of mast cells in microscopic grades II and III compared to grade I indicates the role of mast cells as an indicator of disease progression. This finding may support the use of novel treatment approaches, such as mast cell degranulation inhibitors and anti-angiogenic therapies.
