Volume 28, Issue 169 (2-2019)                   J Mazandaran Univ Med Sci 2019, 28(169): 169-174 | Back to browse issues page

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Jebelli A, Khalaj-Kondori M, Bonyadi M, Hosseinpour Feizi M A, Rahmati-Yamchi M. Effect of the Ethanolic Extract of Olibanum on the Expression of CaMKIIα Gene in B65 and PC12 Cells. J Mazandaran Univ Med Sci 2019; 28 (169) :169-174
URL: http://jmums.mazums.ac.ir/article-1-10281-en.html
Abstract:   (2993 Views)
Background and purpose: In the past, olibanum was used as an incense and perfume and a remedy for treating various diseases. Now, it is confirmed that olibanum improves memory and has a role in treatment and prevention of Alzheimer disease. CaMKIIα gene is known as molecular memory because of its role in cellular processes associated with memory. The aim of this research was to study the effect of ethanolic extract of olibanum on the expression of CaMKIIα in B65 and PC12 cells.
Materials and methods: To determine the effect of olibanum on cell viability, MTT assay was done and cells were treated with the extract in different concentrations and times. To study the gene expression, the cells were treated by two concentrations of the extract at four time points. Then, RNA was extracted, cDNA was synthesized, and the expression of CaMKIIα was quantified by qPCR.
Results: The qPCR data revealed that olibanum alternately changed the expression of CaMKIIα in a reducing and increasing pattern over time in B65 cells. However, the extract could not induce the expression of this gene in PC12 cells.
Conclusion: According to the role of CaMKIIα as molecular memory and the positive effect of olibanum in memory improvement, current results could be helpful in understanding the molecular mechanisms by which olibanum affects memory performance.
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Type of Study: Brief Report | Subject: genetic

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