Volume 33, Issue 222 (7-2023)                   J Mazandaran Univ Med Sci 2023, 33(222): 15-30 | Back to browse issues page

XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Amini Kadijani B, Hashemi B, Mahdavi S R, Soleimani M. Effect of Gold Nanoparticles Conjugated with Folic Acid on the Absorption and Radiosensitization of Cervical Cancer Cells Under 6 MV Photon Irradiation. J Mazandaran Univ Med Sci 2023; 33 (222) :15-30
URL: http://jmums.mazums.ac.ir/article-1-19302-en.html
Abstract:   (1043 Views)
 Background and purpose: Radiation therapy is one of the most important methods in treatment of cancer. Targeted metal nanoparticles (NPs) play an effective role in reducing ionizing radiation side effects through increasing the effect of ionizing radiation on cancer cells and controlling the harmful effect of radiation on healthy cells. The purpose of this study was to investigate the effect of targeted folic acid gold NPs (GNPs) using linker bovine serum albumin in the absorption and sensitivity of gold NPs in HeLa cervical cancer cells compared to non-targeted gold NPs.
Materials and methods: In this study, GNPs were conjugated with folic acid by bovine serum albumin as the linker. The binding of bovine serum albumin and folic acid to GNPs was investigated by infrared (IR) spectroscopy. Toxicity was measured by the MTT method and based on the results, the GNPs’ non-toxic concentration was determined. The effect of targeted synthesized GNPs on their absorption and sensitivity were evaluated on HeLa cells.
Results: The non-toxic concentration of GNPs was 12 μg/ml. Incubation of the cells with this concentration was performed for 2, 4, and 24 hours and it was observed that in 4 hours, the absorption rate of targeted GNPs was about 4 times higher than that of non-targeted GNPs. The radiosensitization ratios of targeted and non-targeted GNPs in the studied cancer cells relative to the control group (without NPs) exposed to 6 MV photon radiation were 1.32±0.02 and 1.19±0.02, respectively.
Conclusion: The use of bovine serum albumin linker to target GNPs has a significant effect
on increasing the absorption of GNPs and the effect of ionizing radiation on HeLa cells under 6 MV photon beams.

 
Full-Text [PDF 688 kb]   (550 Downloads)    
Type of Study: Research(Original) | Subject: Medical physics

Add your comments about this article : Your username or Email:
CAPTCHA

Send email to the article author


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2024 CC BY-NC 4.0 | Journal of Mazandaran University of Medical Sciences

Designed & Developed by : Yektaweb