Volume 35, Issue 247 (8-2025)
J Mazandaran Univ Med Sci 2025, 35(247): 27-39 |
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Aghamardi M, Barzroodipour M, Azarbarz N, Bayatiani M, Bayat M. The Effect of Low-Level Laser Therapy on the Expression of 3β-HSD and StAR Genes in the Ovaries of NMRI Mice. J Mazandaran Univ Med Sci 2025; 35 (247) :27-39
URL: http://jmums.mazums.ac.ir/article-1-21794-en.html
URL: http://jmums.mazums.ac.ir/article-1-21794-en.html
Abstract: (101 Views)
Background and purpose: Ovulation induction increases the number and quality of oocytes, thereby enhancing fertility. However, it is associated with complications such as ovarian hyperstimulation syndrome, apoptosis, and oxidative stress, which may negatively affect oocyte quality. Low-level laser therapy (LLLT), by modulating cellular activity and increasing the expression of genes involved in steroidogenesis, has the potential to improve ovarian function. This study aimed to investigate the effect of LLLT on the expression of 3β-HSD and StAR genes involved in the steroidogenesis pathway, as well as on the number and quality of oocytes and the histological features of ovaries in female NMRI mice.
Materials and methods: In this experimental study, 32 adults female NMRI mice were divided into four groups: control, laser, PMSG, and PMSG & laser. Laser irradiation with a wavelength of 808 nm and a power of 60 mW was applied to the ovaries three times weekly for 21 days. After treatment, ovaries were collected for histological examination and gene expression analysis using real-time PCR. Additionally, the number and quality of oocytes were evaluated, and the data were statistically analyzed.
Results: The results showed that the size of primary and secondary follicles, as well as the expression of 3β-HSD and StAR genes, significantly increased in the PMSG and combined PMSG & laser groups compared to the control and laser groups. The number of atretic follicles significantly decreased in the combined PMSG & laser group compared to the control and PMSG groups. The size of Graafian follicles was significantly larger in the combined group compared to the PMSG and laser groups. Oocyte quality was improved in both the PMSG and combined PMSG & laser groups.
Conclusion: Low-level laser therapy combined with ovulation induction significantly increases the expression of key steroidogenesis genes and improves ovarian histological parameters, thereby enhancing the number and quality of oocytes and overall ovarian function. Therefore, LLLT can be considered a promising complementary approach in infertility treatment.
Materials and methods: In this experimental study, 32 adults female NMRI mice were divided into four groups: control, laser, PMSG, and PMSG & laser. Laser irradiation with a wavelength of 808 nm and a power of 60 mW was applied to the ovaries three times weekly for 21 days. After treatment, ovaries were collected for histological examination and gene expression analysis using real-time PCR. Additionally, the number and quality of oocytes were evaluated, and the data were statistically analyzed.
Results: The results showed that the size of primary and secondary follicles, as well as the expression of 3β-HSD and StAR genes, significantly increased in the PMSG and combined PMSG & laser groups compared to the control and laser groups. The number of atretic follicles significantly decreased in the combined PMSG & laser group compared to the control and PMSG groups. The size of Graafian follicles was significantly larger in the combined group compared to the PMSG and laser groups. Oocyte quality was improved in both the PMSG and combined PMSG & laser groups.
Conclusion: Low-level laser therapy combined with ovulation induction significantly increases the expression of key steroidogenesis genes and improves ovarian histological parameters, thereby enhancing the number and quality of oocytes and overall ovarian function. Therefore, LLLT can be considered a promising complementary approach in infertility treatment.
Keywords: Low-level laser therapy, steroidogenesis, ovary, 3βHSD, StAR, ovulation induction, NMRI mouse
Type of Study: Research(Original) |
Subject:
embryology
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