Volume 24, Issue 112 (5-2014)
J Mazandaran Univ Med Sci 2014, 24(112): 24-32 |
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Mohseni Kouchesfehani H, Saraee F, Maleki M, Nikougoftar M, Khatami S, Sagha M. Evaluation of surface markers and related genes of the human umbilical cord derived Wharton’s jelly mesenchymal stem cells. J Mazandaran Univ Med Sci 2014; 24 (112) :24-32
URL: http://jmums.mazums.ac.ir/article-1-3124-en.html
URL: http://jmums.mazums.ac.ir/article-1-3124-en.html
Homa Mohseni Kouchesfehani 
, Farnoosh Saraee , Masoud Maleki , Mahin Nikougoftar , Seyedeh-Mahsa Khatami , Mohsen Sagha
, Farnoosh Saraee , Masoud Maleki , Mahin Nikougoftar , Seyedeh-Mahsa Khatami , Mohsen Sagha
Abstract: (9483 Views)
Background and purpose: Umbilical cord derived Wharton's jelly is an enriched and accessible source of stem cells with highly proliferative and differentiation potential. This study aimed to evaluate the surface markers and related genes of the stem cells isolated from the human Wharton's jelly.
Materials and methods: Explants of the human umbilical cord derived Wharton's jelly was dissected and cultured in Dulbecco's modified Eagle's medium (DMEM) supplemented with 20% Fetal bovine serum (FBS). Then, those cells migrated from explant's boundary were replated and passaged in DMEM containing 10% FBS. Finally, by using flowcytometry and (Reverse–transcriptase polymerase chain reaction) RT-PCR techniques different surface markers and related genes were analyzed.
Results: 5-7 days post-plating, the stem cells initiated the migration from cultured explants and showed up to 80% densities on days 16-18. Light microscopy demonstrated two distinct cell populations including fibroblast-like and flat endothelial-like cells. Dual staining with flowcytometry also revealed that the cultured cells were found to be positive for CD44, CD73, CD90, CD105 and negative for CD34 and CD45. RT-PCR showed no changes in CD marker expression pattern during different passages.
Conclusion: Human Wharton's jelly derived stem cells appear mesenchymal cell morphology and express related surface markers but no hematopoietic stem cell markers.
Type of Study: Research(Original) |
Subject:
embryology
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