Volume 27, Issue 151 (8-2017)
J Mazandaran Univ Med Sci 2017, 27(151): 32-40 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Tofighi Z, Mohamadi H, Shokrzadeh M, Ghahremani M H, Noori S, Habibi E. Cytotoxic Effect of Hydro-Alcoholic Extract of Crocus caspius on Breast Caner Cell Lines. J Mazandaran Univ Med Sci 2017; 27 (151) :32-40
URL: http://jmums.mazums.ac.ir/article-1-9569-en.html
URL: http://jmums.mazums.ac.ir/article-1-9569-en.html
Zahra Tofighi 
, Hamidreza Mohamadi , Mohammad Shokrzadeh , Mohammad Hosein Ghahremani , Sajad Noori , Emran Habibi
, Hamidreza Mohamadi , Mohammad Shokrzadeh , Mohammad Hosein Ghahremani , Sajad Noori , Emran Habibi
Abstract: (6115 Views)
Background and purpose: Breast cancer is the most common cancer in woman. About one million new cases are diagnosed every year worldwide. Many researchers are interested in anti-cancer effects of natural products. In this research, cytotoxic effects of Crocus caspius extract against breast cancer and normal cell lines were investigated and total phenols and flavonoids were also determined.
Materials and methods: Flowers of Crocus caspius were collected from Neka in Mazandaran province, Iran, in autumn 2014. The powder of dried flowers were macerated with 80% methanol, three times every 48 hr. The methanol extract was concentrated under reduced pressure. Cell viability was determined using MTT and trypan blue assay was done on breast carcinoma cell lines (MCF7, 4T1, and SKBR3) and Swiss mouse embryo fibroblast (NIH/3T3) as normal cell lines. Various concentrations (1, 50, 100, 500, and 1000 µg/ml) of the extract and positive control were examined for determination of IC50.
Results: Crocus caspius extract showed no considerable cytotoxic effects against breast cancer and normal cell lines. Total phenolic and flavonoid contents of the extract were quite high; 238.25±4.35 mg GAE/g and 85.41±6.24 mg quercetin/g of dry extract, respectively.
Conclusion: These results indicated non toxicity of Crocus caspius extract. Further studies are needed to investigate the preventive effect of C.caspius extract against cancer according to its total phenolic and flavonoid contents.
Materials and methods: Flowers of Crocus caspius were collected from Neka in Mazandaran province, Iran, in autumn 2014. The powder of dried flowers were macerated with 80% methanol, three times every 48 hr. The methanol extract was concentrated under reduced pressure. Cell viability was determined using MTT and trypan blue assay was done on breast carcinoma cell lines (MCF7, 4T1, and SKBR3) and Swiss mouse embryo fibroblast (NIH/3T3) as normal cell lines. Various concentrations (1, 50, 100, 500, and 1000 µg/ml) of the extract and positive control were examined for determination of IC50.
Results: Crocus caspius extract showed no considerable cytotoxic effects against breast cancer and normal cell lines. Total phenolic and flavonoid contents of the extract were quite high; 238.25±4.35 mg GAE/g and 85.41±6.24 mg quercetin/g of dry extract, respectively.
Conclusion: These results indicated non toxicity of Crocus caspius extract. Further studies are needed to investigate the preventive effect of C.caspius extract against cancer according to its total phenolic and flavonoid contents.
Type of Study: Research(Original) |
Subject:
Pharmacognosy
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
Articles Copyright © The Author(s). Owned by Mazandaran University of Medical Sciences.
Contact Us
Address: Journal Office, Building No. 2, Mazandaran University of Medical Sciences, Moallem Square, Sari.Tel: +98 (011)34484874 Postal code: 48175-866 E-Mail: jmums@mazums.ac.ir Telefax: +98 (011)33262679
