Volume 35, Issue 248 (9-2025)
J Mazandaran Univ Med Sci 2025, 35(248): 74-81 |
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Akbari F, Azadbakht M, Ebrahimi F, Mahdizadeh Z, Bagheri A, Razavi S. In Vitro Evaluation of the Effects of Astragalus brachycalyx Fisch. Root Methanolic Extract on B16-F10 Cells in Relation to Its Phytochemical Properties. J Mazandaran Univ Med Sci 2025; 35 (248) :74-81
URL: http://jmums.mazums.ac.ir/article-1-21070-en.html
URL: http://jmums.mazums.ac.ir/article-1-21070-en.html
Fatemeh Akbari 
, Mohammad Azadbakht , Fatemeh Ebrahimi , Zahra Mahdizadeh , Ali Bagheri , Setayesh Razavi
, Mohammad Azadbakht , Fatemeh Ebrahimi , Zahra Mahdizadeh , Ali Bagheri , Setayesh Razavi
Abstract: (188 Views)
Background and purpose: Skin cancer is a highly prevalent disease, affecting approximately 1 in 33 individuals with fair skin. Melanoma, one of the most aggressive forms of skin cancer, arises from melanin-producing cells known as melanocytes. This study aims to investigate the effect of Astragalus brachycalyx on melanoma cells in vitro, with a particular focus on its phytochemical properties.
Materials and methods: In this experimental study, the methanolic root extract was prepared using the maceration method, and total flavonoid content was determined by the aluminum chloride colorimetric method. Genistein content was quantified using high-performance liquid chromatography (HPLC). To evaluate the effect of the extract, a scratch assay was performed on B16-F10 cells. Extract concentrations of 125, 62.5, 32.25, and 16.5 ppm were tested in separate culture media and compared with media lacking the extract (control group). Data were analyzed using a two-way mixed-model ANOVA, and multiple comparisons were performed using Bonferroni post hoc analysis. A P-value < 0.05 was considered statistically significant.
Results: The total flavonoid content of Astragalus brachycalyx root was 0.0454 g per gram of root, and the genistein content in the plant powder was 1.1%. Treatment of B16-F10 cells with 500 ppm of the methanolic extract resulted in significant cell death within 24 hours (P < 0.001). Additionally, the extract at 125 ppm caused a significant increase in scratch area (P < 0.0001), indicating inhibition of cell growth compared to the control group (P < 0.001).
Conclusion: The in vitro results demonstrate that Astragalus brachycalyx exhibits rapid and potent cytotoxic effects on B16-F10 melanoma cells
Materials and methods: In this experimental study, the methanolic root extract was prepared using the maceration method, and total flavonoid content was determined by the aluminum chloride colorimetric method. Genistein content was quantified using high-performance liquid chromatography (HPLC). To evaluate the effect of the extract, a scratch assay was performed on B16-F10 cells. Extract concentrations of 125, 62.5, 32.25, and 16.5 ppm were tested in separate culture media and compared with media lacking the extract (control group). Data were analyzed using a two-way mixed-model ANOVA, and multiple comparisons were performed using Bonferroni post hoc analysis. A P-value < 0.05 was considered statistically significant.
Results: The total flavonoid content of Astragalus brachycalyx root was 0.0454 g per gram of root, and the genistein content in the plant powder was 1.1%. Treatment of B16-F10 cells with 500 ppm of the methanolic extract resulted in significant cell death within 24 hours (P < 0.001). Additionally, the extract at 125 ppm caused a significant increase in scratch area (P < 0.0001), indicating inhibition of cell growth compared to the control group (P < 0.001).
Conclusion: The in vitro results demonstrate that Astragalus brachycalyx exhibits rapid and potent cytotoxic effects on B16-F10 melanoma cells
Type of Study: Brief Report |
Subject:
Pharmacognosy
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