Volume 27, Issue 153 (10-2017)                   J Mazandaran Univ Med Sci 2017, 27(153): 1-12 | Back to browse issues page

XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Ranjbaran H, Abediankenari S, Azadbakht M, Khalilian A, Momeninezhad Amiri M, Hosseini S et al . Differentiation of Mesenchymal Stem Cells to Insulin Producing Cells Using the Extracts of Allium ursinum and Silybum marianum. J Mazandaran Univ Med Sci 2017; 27 (153) :1-12
URL: http://jmums.mazums.ac.ir/article-1-10427-en.html
Abstract:   (7820 Views)
Background and purpose: Today, increased rate of demand for insulin is predictable due to increasing cases of diabetics in the world. Therefore, it is necessary to develop economic approaches and increasing the production of insulin for the future and medicinal plants could be regarded as a promising prospect for insulin production.
Materials and methods: The Allium ursinum and Silybum marianum were collected. Each herbarium was identified in School of Pharmacy affiliated with Mazandaran University of Medical Sciences and the extract was used by percolation with 70% ethanol extraction, after the solvent was evaporated by using the rotary. After the successful isolation of mesenchymal stem cells (MSCs), Wharton's jelly was derived and approved. Then, the mesenchymal stem cells were differentiated to pancreatic beta cells with two herbal extracts.
Results: Compared with the control group, there was a significant difference between the levels of insulin in the culture medium obtained from the two plants (P= 0.0001). In addition, via specific dithizone staining, the insulin producing cells (IPCs) were proven.
Conclusion: The extracts of Allium ursinum and Silybum marianum were found capable in inducing differentiation of the mesenchymal stem cells derived from Wharton's jelly into IPCs. Allium ursinum was seen with the highest rate of insulin production, while Silybum marianum had the lowest rate of insulin production, therefore, Allium ursinum could be more effective in treatment of diabetes.
 
Full-Text [PDF 467 kb]   (1716 Downloads)    
Type of Study: Research(Original) | Subject: Pharmacognosy

Add your comments about this article : Your username or Email:
CAPTCHA

Send email to the article author


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2024 CC BY-NC 4.0 | Journal of Mazandaran University of Medical Sciences

Designed & Developed by : Yektaweb