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Background and purpose: Penicilliums have high diversity among fungi species and some of them are found to be very useful. Some studies have evaluated their antimicrobial and cytotoxic effects. Penicillium citrinum is a genus of the penicilliums that produces mycotoxin citrinin. Therefore, it is worthy to assess its cytotoxic effect. Materials and methods: The DNA of the fungus obtained from the soil samples from the campus of Mazandaran University of Medical sciences was extracted. Then DNA sequencing was done and the ethanolic extract including metabolites was taken out. The effect of different concentrations of test solution were evaluated on cancer cell lines of human liver (HepG2), lung (A549), ovary (SKOV3), and breast (MCF7) and also on kidney (LLCPK1) and ovary of Hamster (CHO) normal cell lines using MTT method. Cisplatin was considered as positive control. The data was analyzed using Prism Ver.3, ANOVA and t-test. Results: The findings revealed significant differences between the levels of IC50 of fungus metabolites and cisplatin in all cell lines (P< 0.005). Also, the level of IC50 of fungus metabolites on normal cell lines was significantly different from that of the cancer cell lines (P< 0.05). Conclusion: This study showed that ethanolic extract of P. citrinum metabolites did not have a considerable toxicity effect on cancer and normal cell lines. However, it increaesd the inhibitory effect of cancer cell proliferation.

Keywords: Penicillium citrinum, cancer and normal cell lines, IC50, MTT assay

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