Volume 31, Issue 198 (7-2021)
J Mazandaran Univ Med Sci 2021, 31(198): 40-47 |
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Mousavi T, Valadan R, Rafiei A, Haghshenas M. Examination of MHC-1 Expression by J774 Macrophage Cells Treated with Recombinant Protein HIV-1 Tat 49-59 / HPV16, 18, 6, 11 E7 In Vitro. J Mazandaran Univ Med Sci 2021; 31 (198) :40-47
URL: http://jmums.mazums.ac.ir/article-1-16530-en.html
URL: http://jmums.mazums.ac.ir/article-1-16530-en.html
Abstract: (1293 Views)
Background and purpose: Human papillomavirus (HPV) is one of the infectious agents that causes genital and non-genital warts and skin cancers in humans. The E7 protein of this virus is one of the small oncoproteins that may be the main target in therapeutic vaccines. E7 protein with HIV-1 Tat peptide (49-59), plays a protective role that cause immune Th1 and CTLs response. The aim of this study was to design a recombinant HIV-1 Tat 49-59 / HPV16, 18, 6, 11 E7 protein in vitro and the function of this antigen for MHC-1 expression.
Materials and methods: In this study, macrophage cell line J774 was used to evaluate the expression of MHC-1 and the function of Tat peptide in delivery of recombinant protein to the surface of MHC-1. J744 mouse macrophage cell line was treated by10, 50, and 100 μg of each of the proteins with and without Tat peptide. After 24 hours, the cells were collected and then analyzed by flow cytometry.
Results: In this study, J774 cells were treated and analyzed by E7-Tat and E7 proteins at different concentrations. The study showed that 10 μg of E7-Tat protein increased the expression of MHC-1, while at higher concentrations of this protein, the expression of MHC-1 molecule decreased considerably compared to the group without Tat peptide.
Conclusion: E7-Tat protein at lower concentrations can act as a stimulant for increasing the MHC-1 expression and could be used in therapeutic vaccines.
Materials and methods: In this study, macrophage cell line J774 was used to evaluate the expression of MHC-1 and the function of Tat peptide in delivery of recombinant protein to the surface of MHC-1. J744 mouse macrophage cell line was treated by10, 50, and 100 μg of each of the proteins with and without Tat peptide. After 24 hours, the cells were collected and then analyzed by flow cytometry.
Results: In this study, J774 cells were treated and analyzed by E7-Tat and E7 proteins at different concentrations. The study showed that 10 μg of E7-Tat protein increased the expression of MHC-1, while at higher concentrations of this protein, the expression of MHC-1 molecule decreased considerably compared to the group without Tat peptide.
Conclusion: E7-Tat protein at lower concentrations can act as a stimulant for increasing the MHC-1 expression and could be used in therapeutic vaccines.
Keywords: MHC1 expression, J774 cells, HIV-1 Tat 49-59 peptide, HPV16, 18, 6, 11 E7 recombinant protein, therapeutic vaccine
Type of Study: Research(Original) |
Subject:
Immunology
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