Volume 33, Issue 223 (8-2023)
J Mazandaran Univ Med Sci 2023, 33(223): 11-24 |
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Keshavarz S, Owjfard M, Karbalaei N. Antioxidant Effects of Defrasirox, Defropyrone, and Their Combination on Lead-Induced Nitro-Oxidative Stress
in Male Rats. J Mazandaran Univ Med Sci 2023; 33 (223) :11-24
URL: http://jmums.mazums.ac.ir/article-1-19364-en.html
URL: http://jmums.mazums.ac.ir/article-1-19364-en.html
Abstract: (402 Views)
Background and purpose: Lead poisoning induces oxidative stress and causes disorders in several organs. Iron-chelating drugs can form a complex with toxic metals such as lead and lower their content in the blood and tissues. This study aims to examine the antioxidant effects of Deferasirox, Deferiprone, and their combination in rats with subchronic lead exposure.
Materials and methods: In this interventional study, lead poisoning was induced in Sprague-Dawley male rats by gavage administration of 30 mg/kg lead acetate for 60 days. The animals were treated with Deferasirox (140 mg/kg), Deferiprone (300 mg/kg), and their combination through oral gavage from days 47 to 60 of the experiment (14 days). Lead concentration was measured by flame atomic absorption spectroscopy. We examined malondialdehyde (MDA) level, nitric oxide metabolites (NOx) as nitro-oxidative stress markers, glutathione (GSH), and total antioxidant capacity (TAC) in the blood, brain, liver, kidney, and testis. Data analysis was performed in Graphpad Prism software applying one-way variance analysis and Tukey's test.
Results: Lead poisoning increased the concentration of this metal and nitro-oxidative stress and decreased the TAC and GSH in the brain, liver, kidney, and testis (P<0.05). Alone or in combination, Defropyrone and Defrasirox lowered the lead content in the blood and tissues of rats. In addition, treatment with these two chelators resulted in drops in MDA and NOx levels and increase in TAC and GSH levels (P<0.05), although these effects were most pronounced when the medicines were administered together.
Conclusion: In addition to their antioxidant properties, it seems that Defropyrone and Defrasirox have synergistic effects in lowering lead content in blood and other tissues.
Materials and methods: In this interventional study, lead poisoning was induced in Sprague-Dawley male rats by gavage administration of 30 mg/kg lead acetate for 60 days. The animals were treated with Deferasirox (140 mg/kg), Deferiprone (300 mg/kg), and their combination through oral gavage from days 47 to 60 of the experiment (14 days). Lead concentration was measured by flame atomic absorption spectroscopy. We examined malondialdehyde (MDA) level, nitric oxide metabolites (NOx) as nitro-oxidative stress markers, glutathione (GSH), and total antioxidant capacity (TAC) in the blood, brain, liver, kidney, and testis. Data analysis was performed in Graphpad Prism software applying one-way variance analysis and Tukey's test.
Results: Lead poisoning increased the concentration of this metal and nitro-oxidative stress and decreased the TAC and GSH in the brain, liver, kidney, and testis (P<0.05). Alone or in combination, Defropyrone and Defrasirox lowered the lead content in the blood and tissues of rats. In addition, treatment with these two chelators resulted in drops in MDA and NOx levels and increase in TAC and GSH levels (P<0.05), although these effects were most pronounced when the medicines were administered together.
Conclusion: In addition to their antioxidant properties, it seems that Defropyrone and Defrasirox have synergistic effects in lowering lead content in blood and other tissues.
Type of Study: Research(Original) |
Subject:
physiology
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