Volume 22, Issue 2 (3-2013)                   J Mazandaran Univ Med Sci 2013, 22(2): 92-98 | Back to browse issues page

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Shokrzade M, Abbasi A, Shadborestan A, Omidi M, Jivad F. The Protective role of selenium in preventing tissue damage induced by diazinon in rats.. J Mazandaran Univ Med Sci. 2013; 22 (2) :92-98
URL: http://jmums.mazums.ac.ir/article-1-2103-en.html
Abstract:   (10335 Views)
Background and purpose: Diazinon (DZN) is a commonly used organophos‌phorous (OP) pesticide. It is synthetic chemical substance with broad spectrum insecticide activity [3].Toxic effects of diazinon are due to the inhibition of acetyl cholinesterase activity, an enzyme needed for proper nervous system function. It has been widely used throughout the world with applications in agricul‌ture and horticulture for controlling insects [4,5]. The Se is an essential dietary trace element which plays an important role in a number of biological processes [18] consists of antioxidant and anti mutagenic properties Materials and methods: In the experiment, a total of 45 rats (200-250 gr) were used. The rats were randomly divided into 9 groups with five animals in each group. Animals were concurrently administered intraperitoneally with DZN in proper solvent (20 mg/kg body weight (b.w.)) and Se at three different doses (0.5, 1, and 2 mg/kg b.w.) for 30 consecutive days.After necropsy, liver and kidneys of all animals were isolated and fixed in 10% buffered formalin. Using examples of common practices pathology tissue sections, 5 microns thick sections by microtome and prepared for staining with hematoxylin - eosin were prepared. Results: In the liver of groups that diazinon had received, focal hepatic necrosis was created. Exposure also causes dilation of sinusoids in the liver parenchyma.Selenium in Dose of 0.5mg / kg asthecontrolgroup, although high dose 2mg / kg causesfibrosisandfocalnecrosis, venous congestion and spasticitytic. Ingroups with diazinon received Selenium 0.5 mg / kg lead toinflammatory cellinfiltrationin the portaltract and centralveindilatation.Together with theselenium 2 mg/kgdiazinoncausednecrosisand hepaticnecrosis inzone 3andspasticitytic.However,inthis study,diazinonwasinduced inflammation butnosignificantchangeinkidney of rat’s .necrosisanddestruction ofepithelial cellswere notobserved Conclusion: Thisstudy showed thatdiazinonhas theability to causeliver and kidneydamage, although the damage is less in kidney in comparison with liver damage.It was alsoobservedthatselenium in dose of 0.5 mg / kg is able toreducethedamage however the damage increased when the dose was increasing. It seems , selenium has a harmful role in increased doses, and it result to necrosis and fibrosis
Full-Text [PDF 248 kb]   (1887 Downloads)    
Type of Study: Research(Original) | Subject: toxicology

Add your comments about this article : Your username or Email:

Send email to the article author

© 2020 All Rights Reserved | Journal of Mazandaran University of Medical Sciences

Designed & Developed by : Yektaweb