Volume 34, Issue 239 (11-2024)
J Mazandaran Univ Med Sci 2024, 34(239): 15-25 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Rahmani Chegani S, Rozbahani M, Hosseini S A, Hematfar A, Moini M. The Impact of High Intensity Interval Training and Bee Pollen Supplementation on BDNF Levels in Rats Exposed to Cadmium. J Mazandaran Univ Med Sci 2024; 34 (239) :15-25
URL: http://jmums.mazums.ac.ir/article-1-21121-en.html
URL: http://jmums.mazums.ac.ir/article-1-21121-en.html
Abstract: (816 Views)
Background and purpose: Exposure to heavy metals like cadmium leads to toxicity in the body, and these toxins trigger the production of reactive oxygen species, or free radicals. Even small exposures to these substances can impair brain function. High-intensity interval training (HIIT) and bee pollen have been shown to combat these free radicals. The present study aimed to investigate the effectiveness of HIIT and bee pollen on brain-derived neurotrophic factor (BDNF) levels in rats exposed to cadmium.
Materials and methods: In this experimental study, 25 Sprague-Dawley male rats, 8 weeks old with an average weight of 258 g, were randomly divided into five groups of five: (1) healthy control (HC), (2) cadmium (CD), (3) cadmium + bee pollen (PO), (4) cadmium + training (HIIT) and (5) cadmium + bee pollen + training (HIIT-PO). The cadmium-treated groups (CD, PO, HIIT, and HIIT-PO) were exposed to cadmium administered as CdCl2 at a concentration of 400 mg/kg.L dissolved in water. Additionally, the PO and HIIT-PO groups received a daily oral gavage of 200 mg of bee pollen dissolved in 2.4 cc of normal saline. Prior to the training intervention, a VO2max test was conducted to determine the maximum intensity of the training sessions. Following this, an eight-week HIIT program was performed using the incremental Bedford protocol. Forty-eight hours after the final training and supplementation session, and following a 12-hour fasting period, the rats were anesthetized with ketamine (50 mg/kg) and xylazine (20 mg/kg). The hippocampal tissue of the brain was carefully extracted and BDNF levels were measured using an ELISA kit. The experimental data were analyzed using an independent t-test and two-way analysis of variance (ANOVA).
Results: Cadmium caused a significant decrease in BNDF levels (P≤0.05). Both HIIT training and bee pollen individually demonstrated significant effects, increasing BDNF levels (P≤0.05). However, the interaction between HIIT training and bee pollen did not have a significant effect on BDNF levels in the mice (P≥0.05).
Conclusion: Bee pollen, due to its antioxidant properties, increases BDNF levels in rats exposed to cadmium. Similarly, HIIT training enhances BDNF production in the hippocampus of cadmium-exposed rats exposed by influencing the angiogenesis system. However, the combined interaction of bee pollen and HIIT training does not produce a significant effect on BDNF levels in the hippocampus of these rats.
Materials and methods: In this experimental study, 25 Sprague-Dawley male rats, 8 weeks old with an average weight of 258 g, were randomly divided into five groups of five: (1) healthy control (HC), (2) cadmium (CD), (3) cadmium + bee pollen (PO), (4) cadmium + training (HIIT) and (5) cadmium + bee pollen + training (HIIT-PO). The cadmium-treated groups (CD, PO, HIIT, and HIIT-PO) were exposed to cadmium administered as CdCl2 at a concentration of 400 mg/kg.L dissolved in water. Additionally, the PO and HIIT-PO groups received a daily oral gavage of 200 mg of bee pollen dissolved in 2.4 cc of normal saline. Prior to the training intervention, a VO2max test was conducted to determine the maximum intensity of the training sessions. Following this, an eight-week HIIT program was performed using the incremental Bedford protocol. Forty-eight hours after the final training and supplementation session, and following a 12-hour fasting period, the rats were anesthetized with ketamine (50 mg/kg) and xylazine (20 mg/kg). The hippocampal tissue of the brain was carefully extracted and BDNF levels were measured using an ELISA kit. The experimental data were analyzed using an independent t-test and two-way analysis of variance (ANOVA).
Results: Cadmium caused a significant decrease in BNDF levels (P≤0.05). Both HIIT training and bee pollen individually demonstrated significant effects, increasing BDNF levels (P≤0.05). However, the interaction between HIIT training and bee pollen did not have a significant effect on BDNF levels in the mice (P≥0.05).
Conclusion: Bee pollen, due to its antioxidant properties, increases BDNF levels in rats exposed to cadmium. Similarly, HIIT training enhances BDNF production in the hippocampus of cadmium-exposed rats exposed by influencing the angiogenesis system. However, the combined interaction of bee pollen and HIIT training does not produce a significant effect on BDNF levels in the hippocampus of these rats.
Type of Study: Research(Original) |
Subject:
Sport Physiology
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
Articles Copyright © The Author(s). Owned by Mazandaran University of Medical Sciences.
Contact Us
Address: Journal Office, Building No. 2, Mazandaran University of Medical Sciences, Moallem Square, Sari.Tel: +98 (011)34484874 Postal code: 48175-866 E-Mail: jmums@mazums.ac.ir Telefax: +98 (011)33262679
