Bayat M, Soltani M, Torabi R, baazm M, Barzroodi M. Effect of Low-Level Laser Therapy on the Nrf2/Keap1 Pathway in NMRI Mice with Testicular Torsion. J Mazandaran Univ Med Sci 2025; 35 (249) :101-107
URL:
http://jmums.mazums.ac.ir/article-1-21908-en.html
Abstract: (16 Views)
Background and purpose: Testicular torsion (TT) is a urological emergency characterized by twisting of the spermatic cord, which reduces blood flow and leads to testicular ischemia. Low-level laser therapy (LLLT), an emerging therapeutic approach, may enhance mitochondrial energy production and activate immune responses. The aim of this study was to investigate the effect of LLLT on the protective Nrf2/Keap1 pathway in the testes of NMRI mice with testicular torsion.
Materials and methods: In this experimental study, male NMRI mice were randomly divided into four groups (six mice per group): control (Cont), laser (L), torsion (T), and combined torsion and laser (T&L). Both testes were rotated 720 degrees counterclockwise for 2 hours and then returned to their original positions. Laser treatment (808 nm, 0.03 J/cm², 1 min per session) was applied for 21 days, three sessions per week. MDA and TAC levels, as well as Nrf2 and Keap1 gene expression, were measured by real-time PCR.
Results: In the testicular torsion group, a significant increase in MDA levels and a decrease in TAC were observed (P < 0.001). Low-level laser therapy significantly increased the expression of Nrf2 (P < 0.001) and Keap1 (P < 0.01) genes, reduced MDA levels, and elevated TAC levels (P < 0.001).
Conclusion: Low-level laser therapy administered for three weeks following testicular detorsion may reduce oxidative stress and upregulate genes in the Nrf2/Keap1 pathway in mice, and thus could serve as an adjunctive therapy to mitigate testicular torsion–induced injuries.
Type of Study:
Brief Report |
Subject:
Biology