Volume 35, Issue 250 (11-2025)
J Mazandaran Univ Med Sci 2025, 35(250): 39-50 |
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hossienzadeh M, davoodi M, Saadat F, Abbasi Daloii A. Effects of Aerobic Training and Garlic Extract on Apoptotic Markers and the Expression of miR 1 and miR 133 in the Brain Tissue of Reserpine Induced Parkinsonian Rats. J Mazandaran Univ Med Sci 2025; 35 (250) :39-50
URL: http://jmums.mazums.ac.ir/article-1-22150-en.html
URL: http://jmums.mazums.ac.ir/article-1-22150-en.html
Abstract: (363 Views)
Background and purpose: Parkinson’s disease (PD) is associated with increased neuronal apoptosis, and both exercise and antioxidants have been reported to attenuate this process. This study aimed to investigate the effects of eight weeks of aerobic training (AT) combined with garlic extract (G) administration on apoptotic markers and the expression of miR‑1 and miR‑133 in the brain tissue of Reserpine‑induced Parkinsonian rats.
Materials and methods: In this experimental study, forty male Sprague–Dawley rats with PD induction were randomly assigned to four groups: Parkinsonian control (PD), aerobic training (AT), garlic extract (G), and aerobic training plus garlic extract (AT+G). The aerobic training protocol was conducted for eight weeks, five sessions per week, starting from 15 to 48 minutes of running at 10–24 m/min. The aqueous garlic extract (500 mg/kg body weight) was freshly suspended in distilled water and orally administered by gavage. The mRNA expression levels of Bax, Bcl‑2, miR‑1a, and miR‑133a were measured using RT‑PCR, and motor balance was evaluated using the rotarod test. Data were analyzed using one‑way ANOVA followed by Tukey’s post hoc test (P≤0.05).
Results: In the AT, G, and AT+G groups, Bax, Bax/Bcl‑2 ratio, and miR‑1 were significantly decreased, while Bcl‑2 expression was significantly increased compared with the PD group (P≤0.01). The AT and AT+G groups showed a significant increase in miR‑133a expression, with the AT+G group showing more pronounced responses versus single interventions (P≤0.05).
Conclusion: Combined aerobic training and garlic supplementation exert synergistic anti‑apoptotic effects in the brain of parkinsonian rats, likely via modulation of the Bax/Bcl‑2 ratio and apoptosis‑related microRNAs. This combination may also contribute to improved motor balance and cognitive functions.
Materials and methods: In this experimental study, forty male Sprague–Dawley rats with PD induction were randomly assigned to four groups: Parkinsonian control (PD), aerobic training (AT), garlic extract (G), and aerobic training plus garlic extract (AT+G). The aerobic training protocol was conducted for eight weeks, five sessions per week, starting from 15 to 48 minutes of running at 10–24 m/min. The aqueous garlic extract (500 mg/kg body weight) was freshly suspended in distilled water and orally administered by gavage. The mRNA expression levels of Bax, Bcl‑2, miR‑1a, and miR‑133a were measured using RT‑PCR, and motor balance was evaluated using the rotarod test. Data were analyzed using one‑way ANOVA followed by Tukey’s post hoc test (P≤0.05).
Results: In the AT, G, and AT+G groups, Bax, Bax/Bcl‑2 ratio, and miR‑1 were significantly decreased, while Bcl‑2 expression was significantly increased compared with the PD group (P≤0.01). The AT and AT+G groups showed a significant increase in miR‑133a expression, with the AT+G group showing more pronounced responses versus single interventions (P≤0.05).
Conclusion: Combined aerobic training and garlic supplementation exert synergistic anti‑apoptotic effects in the brain of parkinsonian rats, likely via modulation of the Bax/Bcl‑2 ratio and apoptosis‑related microRNAs. This combination may also contribute to improved motor balance and cognitive functions.
Type of Study: Research(Original) |
Subject:
Sport Physiology
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