Volume 36, Issue 257 (5-2026)
J Mazandaran Univ Med Sci 2026, 36(257): 16-29 |
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Foroutan P, Mohammadi Z F, Nasiri K. The Effect of Chronic Aerobic Training on C3aR1 in the Pancreas of Diabetic Rats. J Mazandaran Univ Med Sci 2026; 36 (257) :16-29
URL: http://jmums.mazums.ac.ir/article-1-22640-en.html
URL: http://jmums.mazums.ac.ir/article-1-22640-en.html
Abstract: (230 Views)
Background and purpose: Type 2 diabetes is associated with chronic inflammation and activation of the complement system, a key component of innate immunity, which may impair pancreatic function. Limited evidence exists regarding the effects of exercise on gene and protein expression of the C3aR1 receptor in pancreatic tissue. This study aimed to investigate the effects of 12 weeks of chronic aerobic training on C3aR1 gene and protein expression in the pancreas of type 2 diabetic rats and its relationship with metabolic indices and brown adipose tissue.
Materials and methods: In this experimental study, 36 male Wistar rats were induced with type 2 diabetes using a two-week high-fat diet followed by an intraperitoneal injection of streptozotocin (30 mg/kg). They were then assigned to four groups: Control, Diabetes, Training, and Diabetes+Training. The training protocol consisted of 12 weeks of treadmill running. Gene expression of pancreatic C3aR1 and brown adipose tissue UCP1 was measured using real-time PCR, while protein expression levels of C3aR1 and UCP1 were assessed by Western blot analysis. Brown adipose tissue morphology was evaluated using histological techniques, and morphometric analysis was performed using ImageJ software.
Results: Diabetes induction significantly increased C3aR1 gene and protein expression in the pancreas, whereas aerobic training markedly decreased these levels. These changes were accompanied by reduced blood glucose levels. In brown adipose tissue, exercise increased UCP1 protein expression and enhanced brown adipocyte cells and mitochondrial content.
Conclusion: Aerobic exercise reduces pancreatic C3aR1 expression and promotes mitochondrial biogenesis in brown adipose tissue. These adaptations suggest that exercise may serve as an effective non-pharmacological intervention to reduce inflammation and improve metabolic health in type 2 diabetes.
Materials and methods: In this experimental study, 36 male Wistar rats were induced with type 2 diabetes using a two-week high-fat diet followed by an intraperitoneal injection of streptozotocin (30 mg/kg). They were then assigned to four groups: Control, Diabetes, Training, and Diabetes+Training. The training protocol consisted of 12 weeks of treadmill running. Gene expression of pancreatic C3aR1 and brown adipose tissue UCP1 was measured using real-time PCR, while protein expression levels of C3aR1 and UCP1 were assessed by Western blot analysis. Brown adipose tissue morphology was evaluated using histological techniques, and morphometric analysis was performed using ImageJ software.
Results: Diabetes induction significantly increased C3aR1 gene and protein expression in the pancreas, whereas aerobic training markedly decreased these levels. These changes were accompanied by reduced blood glucose levels. In brown adipose tissue, exercise increased UCP1 protein expression and enhanced brown adipocyte cells and mitochondrial content.
Conclusion: Aerobic exercise reduces pancreatic C3aR1 expression and promotes mitochondrial biogenesis in brown adipose tissue. These adaptations suggest that exercise may serve as an effective non-pharmacological intervention to reduce inflammation and improve metabolic health in type 2 diabetes.
Type of Study: Research(Original) |
Subject:
Animal physiology
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