Volume 23, Issue 103 (8-2013)                   J Mazandaran Univ Med Sci 2013, 23(103): 11-18 | Back to browse issues page

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Ilami O, Rahimian S H, Kargar M, Jahangiri Sisakht A, Saeedinejad S Z, Hadinia A. Detection of Neisseria Gonorrhoeae and Chlamydia Trachomatis in Patients with Symptomatic Urethritis Using Multiplex PCR, Gram Stain and Urine Culture . J Mazandaran Univ Med Sci 2013; 23 (103) :11-18
URL: http://jmums.mazums.ac.ir/article-1-2493-en.html
Detection of Neisseria Gonorrhoeae and Chlamydia Trachomatis in Patients with Symptomatic Urethritis Using Multiplex PCR, Gram Stain and Urine Culture
Owrang Ilami , Seyyed Hoshang Rahimian , Mohammad Kargar , Alborz Jahangiri Sisakht , Seyyed Zaker Saeedinejad , Abolghasem Hadinia
Abstract:   (12659 Views)
Background and purpose: Chlamydia trachomatis and Neisseria gonorrhoeae are the most common causes of sexually transmitted infections. The aim of this study was detection of N. gonorrhoeae and C. trachomatis in patients with symptomatic urethritis using Multiplex PCR, gram stain and urine culture. Materials and methods: This cross sectional study was conducted in 137 patients with symptomatic urethritis, referring to Yasuj Sahid Mofateh Clinic. After completing a demographic questionnaire, 10-15 ml of first void urine was obtained. After centrifugation of the urine, sediments were used for polymerase chain reaction based on plasmid primers and then cultured on chocolate agar medium. The data was analyzed using descriptive statistics and Chi-square test. Results: The patients included 28 (20%) male and 109 (80%) female. The frequency of infection with C. trachomatis and N. gonorrhoeae was 3.65% and 5.11%, respectively. N. gonorrhoeae was detected in two (7.14%) male and five (4.65%) female and C. trachomatis was observed in two (7.14%) male and three (2.6%) female. No coinfection with C. trachomatis and N. gonorrhoeae was detected. Using culture, gram stain and Multiplex PCR for detection of N. gonorrhoeae we found 5.11, 4.38 and 5.84% positive cases, respectively. Through Multiplex PCR assay for detection of C. trachomatis 3.6% of the cases were found positive. Conclusion: The results of this study showed a relatively low frequency of C. trachomatis and N. gonorrhoeae in Yasuj.
Keywords: C. trachomatis, N. gonorrhoeae, urethritis, polymerase chain reaction
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Type of Study: Research(Original) | Subject: Microbiology
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