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Background and purpose: Brucellosis is a zoonotic disease with worldwide distribution. Humans are usually infected through contact with infected animals or unpasteurized dairy products. We observed contradictory diagnostic value of ELISA and Standard tubal agglutination test (wright) in other studies. Therefore, we designed this study to compare the diagnostic value of ELISA and Standard tubal agglutination test (wright) in patients with positive PCR brucellosis. Material and Methods: We designed a diagnostic study and selected 59 suspected patients and 45 healthy individuals during March 2012 to March 2013 in Razi Hospital at Ghaemshahr City. We did all the tests including: PCR, 2-Mercaptoethanol, Wright, Coombs and Wright and Elisa for all patients and healthy subjects. Clinical manifestation and laboratory data were collected. Data were analyzed by SPSS version 16. Results: The patients’ mean age was 37.98±13.79 years and 71.2% were males. All patients had previous exposure to risk factors. The most common signs were fever (86.4%), sweating (71.2%) and arthralgia (71.2%). On the base of PCR, sensitivity, specificity, positive predict value (PPV), negative predict value (NPV) and the overall accuracy of Wright test was 94.73%, 63.63%, 60%, 95.45% and 75%, Elisa IgM test 57.89%, 77.27%, 59.45%, 76.11% and 70.19% and Elisa IgG test 78.94%, 71.21%, 61.22%, 85.45% and 74%. Conclusion: In our study, Wright test has higher sensitivity, NPV and overall accuracy in comparison with ELISA. In ELISA test, ELISA IgG has higher sensitivity, PPV, NPV and overall accuracy in comparison with ELISA IgM.

Keywords: Brucellosis, wright, Elisa, PCR

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