Volume 24, Issue 114 (7-2014)                   J Mazandaran Univ Med Sci 2014, 24(114): 135-139 | Back to browse issues page

XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Bagherzadeh Khodashahri S, Siadat S D, Rahbar M. Identification and Detection of Invasive Haemophilus Influenzae Type b Strains in Clinical Samples Using Polymerase Chain Reaction. J Mazandaran Univ Med Sci 2014; 24 (114) :135-139
URL: http://jmums.mazums.ac.ir/article-1-3943-en.html
Abstract:   (6926 Views)
Background and purpose: Haemophilus influenzae type b (Hib), is one of the major causes of bacterial meningitis in children younger than five years of age, especially in countries that immunization against Hib is not conducted. In this study we have determined the serotype, encapsulated Haemophilus influenza strains isolated from clinical samples by polymerase chain reaction (PCR) method. Material and Methods: In this study, DNA of H. Influenzae spp was extracted through boiling method. The encapsulated strains were determined by bexA gene primers. The encapsulated strains serotype were then detected using type-specific primer sets "a to f". Results: From the total of 50 strains of the Haemophilus influenzae isolated from clinical specimen, four strains have shown the bex A gene which all of them were encapsulated and all of four isolates have been determined as type b strains. Conclusion: It is well shown that H influenza spp were not detected by routinely culture method in many clinical laboratories. Despite the presence of pathogenic strains of Hib in different clinical specimens, molecular diagnostic such as PCR should be replaced and performed for miss detected bacteria.
Full-Text [PDF 122 kb]   (2281 Downloads)    
Type of Study: Brief Report | Subject: Medical Virology

Add your comments about this article : Your username or Email:
CAPTCHA

Send email to the article author


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2024 CC BY-NC 4.0 | Journal of Mazandaran University of Medical Sciences

Designed & Developed by : Yektaweb