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Background and purpose: The best-known groups of Escherichia coli are B2, B1, A, and D. Previous studies have clearly shown the difference between this group such as resistance to antibiotics, the growth rate, and pathogenicity. Therefore, identifying different groups of E. coli could be of great benefit in curing infections caused by this bacterium. There are some defects in traditional diagnosis methods for E. coli groups, hence, this study aimed at finding a more efficient method. Material and methods: To identify different E. coli groups, after surveying genomic sequence of 60 E. coli and their grouping which was done by silico multiplex PCR method, a polymorphic sequence of homoserine kinase enzyme was selected. Then, 20 unknown samples of E. coli strains were grouped by this sequence and multiplex PCR methods. Results: The 20 unknown samples of E. coli strains were grouped identically in both methods. Conclusion: Identification and grouping of different E. coli strains by sequencing method was found to be more precise than multiplex PCR method.

Keywords: Escherichia coli, multiplex PCR, grouping, homoserine kinase, sequencing

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