Volume 24, Issue 118 (11-2014)                   J Mazandaran Univ Med Sci 2014, 24(118): 45-59 | Back to browse issues page

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bakhtiari Z, shahrooz R, ahmadi A, soltanali F. Protective Effect of Crocin on DNA Damage of Sperm and in Vitro Fertilization (IVF) in Adult Male Mice Treated with Cyclophosphamide . J Mazandaran Univ Med Sci. 2014; 24 (118) :45-59
URL: http://jmums.mazums.ac.ir/article-1-4640-en.html
Abstract:   (5232 Views)
Background and purpose: Oxidative stress induced by cyclophosphamide (CP), a chemotherapeutic agent, causes vulnerability of sperm and declining the fertility power. Then in this study the effect of crocin (saffron extract) on amelioration of these side effects was investigated. Materials and methods: In this study 24 adult male mice were divided in three groups (n=8). Control group received normal saline (0.2 ml/day, IP), CP group received cyclophosphamide (15 mg/kg/week, IP), and CP+Cr group received crocin (200 mg/kg/day, IP) along with CP for 35 days. Then the animals were euthanized and malondialdehyde (MDA) in testis was assayed. After collection of sperm from caudate of epididymis, the rate of DNA damage was calculated by acridine orange staining. After stimulation of ovulation in 72 female adult mice, oocytes were collected and transferred in HTF medium that contained BSA. Then, in-vitro fertilization (IVF) was done with capacitated sperms. The rate of fertilization and primitive embryonic growth were evaluated for 120 hours. Results: The results showed increase in fertilization, two cell embryos and blastocysts in CP+Cr group compared to those of the CP group (P<0.05). Also, the crocin in CP+Cr group prevented the increase of total number of arrested embryos and percentage of arrested embryos type I, II, and III (P<0.05). Decrease in MDA assay and sperms with damaged DNA were observed more in CP+Cr group (P<0.05). Conclusion: This study showed the efficiency of crocin in amelioration of fertility and growth of primitive embryo in animals that received CP.
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Type of Study: Research(Original) | Subject: Biomedical engineering

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