Volume 24, Issue 122 (3-2015)
J Mazandaran Univ Med Sci 2015, 24(122): 23-31 |
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Jabbari Amiri M R, Abastabar M, Shokohi T, Aliali M, Saber S. Frequency of Pneumocystis Jirovecii Colonization in Patients with Respiratory Failure Using Microscopic Methods. J Mazandaran Univ Med Sci 2015; 24 (122) :23-31
URL: http://jmums.mazums.ac.ir/article-1-5230-en.html
URL: http://jmums.mazums.ac.ir/article-1-5230-en.html
Abstract: (9244 Views)
Background and purpose: Respiratory failures are common diseases among different populations. Pneumocystis jirovecii colonization causes respiratory failure in children and adults with AIDS and patients with predisposing factors of immunosuppression including malignancies, organ transplant, inherited immune deficiencies and immunosuppressive agents. The aim of this study was to evaluate the efficacy of three staining methods including Calcofluor White, Giemsa and Gomori Methenamine Silver in identification of Pneumosystis colonization using Bronchoalveolar lavage (BAL).
Materials and methods: A cross sectional descriptive study was performed in 350 patients with respiratory failure who referred to Tehran Shariati Hospital and Sari Imam Khomeini Hospital during August 2013 to September 2014. Totally, 322 BAL samples, 26 induced sputa and 2 samples of sinus washing were evaluated for Pneumocystis jirovecii colonization after staining with the three specific staining methods. Data was then analyzed in SPSS V.16.
Results: The patients were 41.2% female and 58.8% male. The mean age of patients was 52 ± 4 years. Calcofluor White staining showed Pneumocystis jirovecii colonization in three patients (0.86%).
Conclusion: In this study, Calcofluor White staining presented higher efficacy in identifying Pneumocystis jirovecii in BAL samples compared to other two microscopic procedures.
Keywords: Pneumocystis jirovecii Colonization, Calcofluor White, Giemsa, Gomori Methenamine Silver, Bronchoalveolar lavage, induced sputum, sinus washing
Type of Study: Research(Original) |
Subject:
mycology
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