Volume 25, Number 123 (4-2015)                   J Mazandaran Univ Med Sci 2015, 25(123): 21-31 | Back to browse issues page


XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Tabatabaei Qomi R, Sheykhhasan M, Kalhor N, Ghiasi M. Chondrogenic Differentiation of Human Adipose-derived Mesenchymal Stem Cells Using Fibrin Hydrogel Scaffold. J Mazandaran Univ Med Sci. 2015; 25 (123) :21-31
URL: http://jmums.mazums.ac.ir/article-1-5517-en.html

Abstract:   (2890 Views)
Background and purpose: Mesenchymal stem cells (MSCs) are the most widely used cell sources for cartilage tissue engineering. In the present study, human stem cells were used as a cell source. Scaffolds play an important role in tissue engineering, therefore, this study aimed at evaluating the ability of fibrin scaffolds in chondrogenic differentiation of adipose-derived mesenchymal stem cells (ADMSCs). Materials and methods: In this study, after preparing fibrin scaffold, isolating mesenchymal stem cells from adipose tissue, and confirming their isolation the cells were cultured into fibrin scaffolds. Seven days after cell culture, their potential of survival and growth were evaluated using MTT assay. Finally, 14 days after the end of the chondrogenic differentiation, gene expression analysis and chondrocyte morphology formation was performed by Real time PCR and histology analysis, respectively. Results: The mesenchymal stem cell viability, proliferation and expression of 4 specific chondrogenic genes significantly increased in a fibrin scaffold compared with those of the control group. Moreover, the formation of chondrocyte cells on fibrin scaffold was confirmed. Conclusion: Fibrin scaffold was found suitable for condrogenic differentiation of adipose-derived mesenchymal stem cells.
Full-Text [PDF 690 kb]   (1167 Downloads)    
Type of Study: Research(Original) | Subject: Biotechnology

Add your comments about this article : Your username or email:
Write the security code in the box

Send email to the article author


© 2015 All Rights Reserved | Journal of Mazandaran University of Medical Sciences

Designed & Developed by : Yektaweb