Volume 25, Number 128 (9-2015)                   J Mazandaran Univ Med Sci 2015, 25(128): 37-46 | Back to browse issues page


XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Sabzikar E, Yari F, Ghasemzadeh M. Effects of Platelet-derived Microparticles on the Respiratory Burst in Neutrophils. J Mazandaran Univ Med Sci. 2015; 25 (128) :37-46
URL: http://jmums.mazums.ac.ir/article-1-6118-en.html

Abstract:   (2045 Views)
Background and purpose: Platelet concentrates (PCs) are used in treatment of quantitative and qualitative platelet defects. In some cases it can cause complications in recipients. In PC, white blood cells, some mediators, cytokines and microparticles can contribute to adverse reactions in recipients. In this study, the effects of platelet-derived microparticles (PMP) were surveyed in the stimulation and activation of neutrophils. Materials and methods: In this experimental study, PC units were prepared from Iranian Blood Transfusion Organization and sampling of the bags was carried out. PMPs were separated from PCs and treated with neutrophils. After the incubation time, the respiratory burst of neutrophils was evaluated by flow cytometry using Dihydrorhodamine 123. Finally, data was analyzed using the Wilcoxon non- parametric test. Results: This study revealed that PMPs were able to stimulate and activate neutrophils (P ≤0.05). This effect depended on the final concentration of PMPs. On the other hand, the expression of CD40L molecules on PMPs showed no significant differences in the studied days during storage. Conclusion: According to this study PMPs in PC could play a role in activation of neutrophils.
Full-Text [PDF 324 kb]   (662 Downloads)    
Type of Study: Research(Original) | Subject: Hematology and Oncology

Add your comments about this article : Your username or email:
Write the security code in the box

Send email to the article author


© 2015 All Rights Reserved | Journal of Mazandaran University of Medical Sciences

Designed & Developed by : Yektaweb