Background and purpose: Hydatid cyst is a zoonotic world-wide infection, caused by larval stage of Echinococcus granulosus. Its mean infection rate in Iran is 1.2 surgical case per 100,000. Hydatidosis is of great economic and medical importance. So, this study was designed to determine the seroprevalence of human hydatidosis using ELISA.

Materials and methods: In this cross-sectional study, 536 blood samples were collected from Shahrebabak residents in 2014. Random cluster sampling was applied to select the participants. Demographic information including age, sex, job, history of contact with dog, and place of residence (city or village) were collected using a questionnaire. The samples were transferred to laboratory and investigated by ELISA. Data was analyzed in SPSS applying and Chi-square test.

Results: Only one male (0.18%) was found to be positive (23 years of age) who was living in city, self-employed and had no history of contact with dog.

Conclusion: According to this study, the prevalence of human hydatid cyst was lower than other regions in Iran.