Volume 26, Issue 140 (9-2016)                   J Mazandaran Univ Med Sci 2016, 26(140): 127-136 | Back to browse issues page

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Tayybi T, Jorfi S, Ghaffari S, Kujlu R. Bioremediation of n-Hexadecane Contaminated Soils Using Pseudomonas aeruginosa Bacteria Isolated from Coastal Areas. J Mazandaran Univ Med Sci. 2016; 26 (140) :127-136
URL: http://jmums.mazums.ac.ir/article-1-8377-en.html
Abstract:   (2808 Views)

Background and purpose: Soil pollution to hydrocarbons in oil field zones in southern Iran and areas close to oil and gas transportation pipe lines is a major environmental problem. The current study aimed at determining the efficiency of bioremediation of n-Hexadecane contaminated soil as a model contaminant.

Materials and methods: Aged hydrocarbon contaminated soils were used as the origin of bacteria isolation. Isolation of n-Hexadecane degrading bacteria was carried out through enrichment in mineral salt medium and incubation in 31 oC in shaker incubator at 150 rpm by repetitive cultures during 6 consecutive refreshing. Finally, two pure strains were isolated and identified by PCR. Bioremediation of initial n-Hexadecane concentrations of 500, 1000 and 5000 mg/kg was investigated in eight weeks.

Results: Two Pseudomonas aeruginosa strains capable of n-Hexadecane biodegradation were isolated. The removal efficiencies at the end of day 56 for n-Hexadecane initial concentrations of 500, 1000 and 5000 mg/kg were 93.6%, 87.5%, and 69.62%, respectively.

Conclusion: Isolated Pseudomonas aeruginosa bacteria demonstrated high n-Hexadecane biodegradation potential in soil and also biosurfactant production, therefore, they could be used for bioremediation of hydrocarbon contaminated soils.

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Type of Study: Research(Original) | Subject: Environmental Health

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