Volume 28, Issue 161 (6-2018)
J Mazandaran Univ Med Sci 2018, 28(161): 115-120 |
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Shokrzadeh M, Rahmati Kukandeh M, Kargar Darabi N, Modanloo M, Fallah M, Mohammadpour A. Cellular Effects of Naringin in Prevention of Genotoxicity Caused by Mifepristone on Human Blood Lymphocytes. J Mazandaran Univ Med Sci 2018; 28 (161) :115-120
URL: http://jmums.mazums.ac.ir/article-1-10746-en.html
URL: http://jmums.mazums.ac.ir/article-1-10746-en.html
Mohammad Shokrzadeh 
, Mahboube Rahmati Kukandeh , Narges Kargar Darabi , Mona Modanloo , Marjan Fallah , Abass Mohammadpour
, Mahboube Rahmati Kukandeh , Narges Kargar Darabi , Mona Modanloo , Marjan Fallah , Abass Mohammadpour
Abstract: (4781 Views)
Background and purpose: Mifepristone is an oral synthetic steroid with antiglucocorticoid and antiprogesterone activities. Therefore, it could be dangerous during pregnancy since it causes cytogenetic damage and, consequently, abortion. Flavonoid compounds such as naringin have antioxidant properties and eliminate various free radicals. Naringin is abundantly found in Iran, so, we investigated its cellular effects against cytogenetic damage caused by mifepristone on blood lymphocyte using micronucleus method.
Materials and Methods: In this experimental study, 5ml Venous blood were collected from 5 healthy and non-smokers using heparin syringe. Different naringin concentrations followed by 43µg/ml damage dose of mifepristone were injected to the cells. To evaluate the production of micronucleus in restrained binucleated lymphocytes in cytokine, the slides were prepared and evaluated by optical microscopy. The mean values were compared applying ANOVA test (posttest: Tukey) in SPSS and p<0.05 was regarded significant.
Results: The highest frequency of micronucleus was observed in the positive control group (17.67±1.528) and the lowest was seen in the naringin group at 232µg/ml (1.33+0.5774) (P<0.05). Naringin (232µg/ml) with mifepristone led to significant changes in the number of micronucleus nuclei compared to the sample without naringin.
Conclusion: Naringin is a potent antigenotoxin against the damage caused by mifepristonethan, therefore, it could be used as a protective agent against the toxic effects of mifepristone.
Materials and Methods: In this experimental study, 5ml Venous blood were collected from 5 healthy and non-smokers using heparin syringe. Different naringin concentrations followed by 43µg/ml damage dose of mifepristone were injected to the cells. To evaluate the production of micronucleus in restrained binucleated lymphocytes in cytokine, the slides were prepared and evaluated by optical microscopy. The mean values were compared applying ANOVA test (posttest: Tukey) in SPSS and p<0.05 was regarded significant.
Results: The highest frequency of micronucleus was observed in the positive control group (17.67±1.528) and the lowest was seen in the naringin group at 232µg/ml (1.33+0.5774) (P<0.05). Naringin (232µg/ml) with mifepristone led to significant changes in the number of micronucleus nuclei compared to the sample without naringin.
Conclusion: Naringin is a potent antigenotoxin against the damage caused by mifepristonethan, therefore, it could be used as a protective agent against the toxic effects of mifepristone.
Type of Study: Brief Report |
Subject:
genetic
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