Volume 28, Issue 169 (2-2019)                   J Mazandaran Univ Med Sci 2019, 28(169): 1-13 | Back to browse issues page

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Mahdavi M, Azadbakht M, Vahdati A, shokrzadeh M, Farhadi A, Davoodi A. Anticancer Effects of Senecionine and Senecio vulgaris L. on Prostate Cancer Cell Lines . J Mazandaran Univ Med Sci. 2019; 28 (169) :1-13
URL: http://jmums.mazums.ac.ir/article-1-12343-en.html
Abstract:   (2034 Views)
Background and purpose: This research aimed at evaluating the effects of senecionine and Senecio vulgaris extract on apoptosis and cell cycle inhibition. Also, their cytotoxicity on prostate cancer cell lines (PC3, DU145 and HGFs), antioxidant effects, and influences on AR and CLU gene expression were investigated.
Materials and methods: In this experimental study, senecionine and S. vulgaris extract were prepared and the induced morphological changes on cell lines were evaluated in DMEM F12 cell culture after 24, 48, 72, and 96 hours by an invert microscope. Then, cell viability and apoptosis were determined by MTT and fluorescence analysis, respectively. Finally, AR and CLU gene expression and methylation of AR gene were evaluated using Real-time PCR and Bi-Sulfide test, respectively.
Results: Cytotoxicity data showed morphological changes 24 hours after treatment with senecionine in all cell lines and all concentrations which also increased in longer time. Based on MTT results, senecionine showed cytotoxicity in 50 and 500 μg/ml on PC3 and du145 prostate cancer cell lines.
Conclusion: According to current findings, Ragwort aerial part extract are suggested for in vivo studies with minimum destruction on normal cells and inducing effects on destruction and apoptosis of prostate carcinogenic cell lines.
Full-Text [PDF 1022 kb]   (711 Downloads)    
Type of Study: Research(Original) | Subject: Pharmacy

Add your comments about this article : Your username or Email:

Send email to the article author

© 2020 All Rights Reserved | Journal of Mazandaran University of Medical Sciences

Designed & Developed by : Yektaweb