Volume 29, Issue 173 (6-2019)
J Mazandaran Univ Med Sci 2019, 29(173): 33-39 |
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Hashemi R, Ataee R A, Alishiri G H, Ghorbanalizadegan M, Mahabadi M, Najafi A. Molecular Detection of Bacterial Etiology of Rheumatoid Arthritis . J Mazandaran Univ Med Sci 2019; 29 (173) :33-39
URL: http://jmums.mazums.ac.ir/article-1-12459-en.html
URL: http://jmums.mazums.ac.ir/article-1-12459-en.html
Reza Hashemi 
, Ramezan Ali Ataee , Gholam Hossein Alishiri , Mahdi Ghorbanalizadegan , Mostafa Mahabadi , Ali Najafi
, Ramezan Ali Ataee , Gholam Hossein Alishiri , Mahdi Ghorbanalizadegan , Mostafa Mahabadi , Ali Najafi
Abstract: (3049 Views)
Background and purpose: Etiology of rheumatoid arthritis is not fully recognized. The purpose of this study was to use universal and specific primers to trace bacteria in the blood and synovial fluid in patients with rheumatoid arthritis.
Materials and methods: In this experimental study, a PCR method was developed to identify a wide range of bacteria in general and Staphylococcus aureus in specific. Ninety five synovial fluid and 100 blood samples being stored at -80°C were assayed. Genome extraction was performed. Universal primer pairs were used for amplification of 16SrRNA and a specific primer was used for nuc gene of Staphylococcus aureus. Then, the PCR product of the specific primer was sequenced and data were analyzed.
Results: The samples of synovial fluid and blood samples of rheumatoid arthritis patients which were negative in bacteriological culture showed 33 (34%) and 36 (36%) cases to be positive for 16Sr RNA, respectively. Also, 21 cases of synovial fluid and only 1 blood sample were positive for Staphylococcus aureus.
Conclusion: The results showed the presence of 16SrRNA gene of different bacteria, including Staphylococcus aureus in blood and synovial fluid of patients. Based on current findings, it is likely to explain a part of the etiology of rheumatoid arthritis, thereby modifying some treatment protocols.
Materials and methods: In this experimental study, a PCR method was developed to identify a wide range of bacteria in general and Staphylococcus aureus in specific. Ninety five synovial fluid and 100 blood samples being stored at -80°C were assayed. Genome extraction was performed. Universal primer pairs were used for amplification of 16SrRNA and a specific primer was used for nuc gene of Staphylococcus aureus. Then, the PCR product of the specific primer was sequenced and data were analyzed.
Results: The samples of synovial fluid and blood samples of rheumatoid arthritis patients which were negative in bacteriological culture showed 33 (34%) and 36 (36%) cases to be positive for 16Sr RNA, respectively. Also, 21 cases of synovial fluid and only 1 blood sample were positive for Staphylococcus aureus.
Conclusion: The results showed the presence of 16SrRNA gene of different bacteria, including Staphylococcus aureus in blood and synovial fluid of patients. Based on current findings, it is likely to explain a part of the etiology of rheumatoid arthritis, thereby modifying some treatment protocols.
Type of Study: Research(Original) |
Subject:
Microbiology
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