Volume 33, Issue 2 (12-2023)
J Mazandaran Univ Med Sci 2023, 33(2): 281-290 |
Back to browse issues page
Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Yazdian-Robati R, Asghari Ayask Z, Arabzadeh S, Hashemi M, Kalalinia F. Evaluation the Effects of both Aqueous and Alcoholic Extract Derived from the Root of Rubia Tinctorum on the Osteogenic Differentiation of Rat Mesenchymal Stem Cells. J Mazandaran Univ Med Sci 2023; 33 (2) :281-290
URL: http://jmums.mazums.ac.ir/article-1-19965-en.html
URL: http://jmums.mazums.ac.ir/article-1-19965-en.html
Rezvan Yazdian-Robati 
, Zeinab Asghari Ayask , Sepideh Arabzadeh , Maryam Hashemi , Fatemeh Kalalinia
, Zeinab Asghari Ayask , Sepideh Arabzadeh , Maryam Hashemi , Fatemeh Kalalinia
Abstract: (320 Views)
Background and purpose: The osteogenic differentiation of MSCs plays a key role in bone tissue engineering and regenerative medicine, making it essential to explore natural compounds that may enhance this process. This aim this study was to investigate the osteogenic potential of both aqueous and alcoholic extracts derived from the root of Rubia tinctorum on rat bone marrow-derived mesenchymal stem cells (BM-MSCs).
Materials and methods: Aqueous and alcoholic extracts were prepared from the root of Rubia tinctorum. Rat BM-MSCs were isolated and cultured and their osteogenic differentiation was assessed in the presence of varying concentrations of the extracts using alizarin red staining and alkaline phosphatase (ALP) activity. Cytotoxic effect of root extract of Rubia tinctorum was evaluated using MTT assay.
Results: The MTT assay demonstrated that the root extract of Rubia tinctorum was cytotoxic at 1000 µg/ml. Additionally, the alizarin red assay showed that the most significant color intensity at 1 and 10 µg/ml concentrations on the 14th day. In terms of bone differentiation induction which measured via the alkaline phosphatase test, both the aqueous and alcoholic extracts at 1 and 10 µg/ml concentrations led to substantial increases (14.9- and 14.57-fold for the aqueous extract and approximately 7.37- and 7-fold for the alcoholic extract, respectively) in alkaline phosphatase activity compared to the negative control.
Conclusion: This study sheds light on the osteogenic effects of Rubia tinctorum root extracts, emphasizing their potential as natural agents in promoting bone regeneration and tissue engineering applications. Further investigations into the underlying molecular mechanisms and in vivo studies are warranted to comprehensively evaluate the feasibility of utilizing these extracts for therapeutic bone regeneration purposes
Materials and methods: Aqueous and alcoholic extracts were prepared from the root of Rubia tinctorum. Rat BM-MSCs were isolated and cultured and their osteogenic differentiation was assessed in the presence of varying concentrations of the extracts using alizarin red staining and alkaline phosphatase (ALP) activity. Cytotoxic effect of root extract of Rubia tinctorum was evaluated using MTT assay.
Results: The MTT assay demonstrated that the root extract of Rubia tinctorum was cytotoxic at 1000 µg/ml. Additionally, the alizarin red assay showed that the most significant color intensity at 1 and 10 µg/ml concentrations on the 14th day. In terms of bone differentiation induction which measured via the alkaline phosphatase test, both the aqueous and alcoholic extracts at 1 and 10 µg/ml concentrations led to substantial increases (14.9- and 14.57-fold for the aqueous extract and approximately 7.37- and 7-fold for the alcoholic extract, respectively) in alkaline phosphatase activity compared to the negative control.
Conclusion: This study sheds light on the osteogenic effects of Rubia tinctorum root extracts, emphasizing their potential as natural agents in promoting bone regeneration and tissue engineering applications. Further investigations into the underlying molecular mechanisms and in vivo studies are warranted to comprehensively evaluate the feasibility of utilizing these extracts for therapeutic bone regeneration purposes
Keywords: Rubia tinctorum, osteogenic differentiation, mesenchymal stem cells, natural extracts, bone tissue engineering
Type of Study: Research(Original) |
Subject:
Nano technology
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
Articles Copyright © The Author(s). Owned by Mazandaran University of Medical Sciences.
Contact Us
Address: Journal Office, Building No. 2, Mazandaran University of Medical Sciences, Moallem Square, Sari.Tel: +98 (011)34484874 Postal code: 48175-866 E-Mail: jmums@mazums.ac.ir Telefax: +98 (011)33262679
