Volume 35, Issue 250 (11-2025)
J Mazandaran Univ Med Sci 2025, 35(250): 3-14 |
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Maleki E, Zargari M, Mirzaei M, Karimpour Malakshah A A, Talebpour Amiri F. Evaluation of the effect of exposure to N-nitrosodiethylamine on rat lung and intestine and the protective effect of tyrosol: A Biochemical and Histopathological study. J Mazandaran Univ Med Sci 2025; 35 (250) :3-14
URL: http://jmums.mazums.ac.ir/article-1-22154-en.html
URL: http://jmums.mazums.ac.ir/article-1-22154-en.html
Elahe Maleki 
, Mehryar Zargari , Mansooreh Mirzaei , Abbas Ali Karimpour Malakshah , Fereshteh Talebpour Amiri
, Mehryar Zargari , Mansooreh Mirzaei , Abbas Ali Karimpour Malakshah , Fereshteh Talebpour Amiri
Abstract: (21 Views)
Background and purpose: N-nitrosodiethylamine (NDEA) is a carcinogenic substance found in various foods. This toxic compound induces oxidative stress, resulting in damage to the structure of several internal organs, particularly the gastrointestinal and respiratory systems. Tyrosol (Tyr), a stable natural flavonoid and a primary component of olive oil, exhibits strong antioxidant properties. This study aims to investigate the protective effects of Tyrosol against NDEA-induced tissue damage in the lung and intestine.
Materials and methods: In this experimental study, 36 adult female rats were randomly divided into six groups: control, Tyr at doses of 10 and 20 mg/kg (administered by gavage for seven consecutive days), NDEA at a dose of 200 mg/kg (single dose on day four), and NDEA combined with Tyr at 10 and 20 mg/kg. Histopathological examinations of lung and jejunal tissue structure changes and biochemical analyses of oxidative stress parameters were performed on day nine. Data were analyzed with one-way analysis of variance (ANOVA) followed by Tukey’s post-hoc test.
Results: Histopathological findings revealed that NDEA exposure caused inflammatory cell infiltration, alveolar wall thickening, collapse of some alveolar sacs, and edema in lung tissue. In the jejunum, administration of NDEA resulted in hemorrhage, infiltration of inflammatory cells within the submucosal layer, and a reduction in goblet cell density. Furthermore, biochemical analyses showed that NDEA induced oxidative stress by reducing GSH levels and elevating MDA concentrations. Tyr administration at both 10 and 20 mg/kg preserved the tissue structure of the lungs and jejunum, with the 20 mg/kg dose exhibiting a more significant protective effect.
Conclusion: The results indicate that Tyr, owing to its antioxidant properties and its ability to modulate oxidative stress parameters, may potentially prevent structural damage in pulmonary and jejunal tissues caused by NDEA.
Materials and methods: In this experimental study, 36 adult female rats were randomly divided into six groups: control, Tyr at doses of 10 and 20 mg/kg (administered by gavage for seven consecutive days), NDEA at a dose of 200 mg/kg (single dose on day four), and NDEA combined with Tyr at 10 and 20 mg/kg. Histopathological examinations of lung and jejunal tissue structure changes and biochemical analyses of oxidative stress parameters were performed on day nine. Data were analyzed with one-way analysis of variance (ANOVA) followed by Tukey’s post-hoc test.
Results: Histopathological findings revealed that NDEA exposure caused inflammatory cell infiltration, alveolar wall thickening, collapse of some alveolar sacs, and edema in lung tissue. In the jejunum, administration of NDEA resulted in hemorrhage, infiltration of inflammatory cells within the submucosal layer, and a reduction in goblet cell density. Furthermore, biochemical analyses showed that NDEA induced oxidative stress by reducing GSH levels and elevating MDA concentrations. Tyr administration at both 10 and 20 mg/kg preserved the tissue structure of the lungs and jejunum, with the 20 mg/kg dose exhibiting a more significant protective effect.
Conclusion: The results indicate that Tyr, owing to its antioxidant properties and its ability to modulate oxidative stress parameters, may potentially prevent structural damage in pulmonary and jejunal tissues caused by NDEA.
Type of Study: Research(Original) |
Subject:
Sports biomechanics
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