Volume 35, Issue 251 (12-2025)
J Mazandaran Univ Med Sci 2025, 35(251): 53-64 |
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Aghaei-Zarch S M, Mahmoudieh L, Miryounesi M, Movafagh A. Investigation of the Expression of lncRNA HOTAIR and lncRNA PVT1 in Individuals with Diabetic Nephropathy. J Mazandaran Univ Med Sci 2025; 35 (251) :53-64
URL: http://jmums.mazums.ac.ir/article-1-22160-en.html
URL: http://jmums.mazums.ac.ir/article-1-22160-en.html
Abstract: (29 Views)
Background and purpose: Diabetic nephropathy (DN) is a major microvascular complication of type 2 diabetes mellitus (T2DM) that can lead to kidney failure. In this study, the expressions of the lncRNA HOTAIR and PVT1 genes were investigated in individuals with type 2 diabetes with DN, individuals with type 2 diabetes without DN, pre-diabetic individuals, and healthy controls. The study also examined the expression levels of lncRNA HOTAIR and PVT1 in HEK-293 cells treated with high glucose (HG) and normal glucose (NG).
Materials and methods: In this cross-sectional study, the expression levels of the lncRNA HOTAIR and PVT1 genes were compared among four groups: 50 healthy individuals, 50 individuals with prediabetes, 50 patients with T2DM, and 50 patients with DN. To model DN in vitro, HEK-293 cells were cultured under high-glucose (HG; 100 mmol/L) and normal-glucose (NG) conditions for 72 hours, and the expression levels of HOTAIR and PVT1 were measured in the treated cells.
Results: The current work showed that the expression level of lncRNA PVT1 was significantly increased in individuals with DN compared with the healthy and prediabetic groups, but there was no significant difference when compared with the T2DM group. The analysis also indicated that the expression level of lncRNA HOTAIR was elevated in the DN group compared with the other groups. Under high-glucose (HG) conditions, an increase in lncRNA PVT1 expression was observed, while no change was detected in the expression level of lncRNA HOTAIR.
Conclusion: The results of this study suggest that lncRNA HOTAIR and lncRNA PVT1 play roles in the pathogenesis of DN, with lncRNA PVT1 showing a stronger association with glucose-induced cellular changes than lncRNA HOTAIR
Materials and methods: In this cross-sectional study, the expression levels of the lncRNA HOTAIR and PVT1 genes were compared among four groups: 50 healthy individuals, 50 individuals with prediabetes, 50 patients with T2DM, and 50 patients with DN. To model DN in vitro, HEK-293 cells were cultured under high-glucose (HG; 100 mmol/L) and normal-glucose (NG) conditions for 72 hours, and the expression levels of HOTAIR and PVT1 were measured in the treated cells.
Results: The current work showed that the expression level of lncRNA PVT1 was significantly increased in individuals with DN compared with the healthy and prediabetic groups, but there was no significant difference when compared with the T2DM group. The analysis also indicated that the expression level of lncRNA HOTAIR was elevated in the DN group compared with the other groups. Under high-glucose (HG) conditions, an increase in lncRNA PVT1 expression was observed, while no change was detected in the expression level of lncRNA HOTAIR.
Conclusion: The results of this study suggest that lncRNA HOTAIR and lncRNA PVT1 play roles in the pathogenesis of DN, with lncRNA PVT1 showing a stronger association with glucose-induced cellular changes than lncRNA HOTAIR
Type of Study: Research(Original) |
Subject:
genetic
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