Volume 24, Issue 112 (5-2014)                   J Mazandaran Univ Med Sci 2014, 24(112): 34-40 | Back to browse issues page

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Hamounnavard S, Delirezh N, Afza Ahangaran N. Evaluation of peripheral blood neutrophil survival following ‎treatment with Mesenchymal stem cells in rats. J Mazandaran Univ Med Sci. 2014; 24 (112) :34-40
URL: http://jmums.mazums.ac.ir/article-1-3056-en.html
Abstract:   (5594 Views)
Background and purpose: Because of theirmultipotency and ease of purification, amplification and ‎immunomodulatory properties Mesenchymal stem cells (MSC), are anideal stem cell source for cell therapies. ‎This study was done to investigat the effect of Rat MSCs on the survival neutrophils.‎ Materials and methods: MSCs was isolated from rat (6-8 weeks) femoral and tibial bone marrow and ‎cultured in DMEM. Then maturation MSCs were incubated with neutrophils isolated from peripheral blood of ‎rat at 37 ° C for 1 h. Neutrophil survival was measured at 6 and 24 h incubation with of MSCs by flow ‎cytometric analysis using An/PI. Data were analyzed by SPSSsoftware, followed by Tukeytest at a significance ‎level of (P<0.05).‎ Results: The 6-hour incubation of neutrophils with MSCs, significantly reduced the percentage of ‎normal cells and increased apoptosis compared to controls and increased cell necrosis was not significant in ‎the treated group than in control. The 24-hour incubation of neutrophils with MSCs, significantly increased the ‎percentage of healthy cells and apoptosis was reduced compared to the control group, and reduced cell ‎necrosis was not significant in the treated groups than in control (p<0.05).‎ Conclusion: In spite of the clinical importance of MSCs, some biological aspects of them including ‎their self-renewal, proliferation and immune modulatory effects are of great therapeutic potential for cell ‎therapy.‎
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Type of Study: Research(Original) | Subject: Immunology

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