Survey on the Association of CCR5 Promoter -59353T/C Polymorphism with HCV Infection - Journal of Mazandaran University of Medical Sciences
Volume 23, Number 109 (2-2014)                   J Mazandaran Univ Med Sci 2014, 23(109): 46-53 | Back to browse issues page


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Hosseini A, Sharifi Z, Baghbani-arani F. Survey on the Association of CCR5 Promoter -59353T/C Polymorphism with HCV Infection. J Mazandaran Univ Med Sci. 2014; 23 (109) :46-53
URL: http://jmums.mazums.ac.ir/article-1-3378-en.html

Abstract:   (4202 Views)
Background and purpose: Hepatitis C is an acute or chronic liver disease worldwide. Chemokines and chemokine receptors are involved in the effective immune response to hepatitis C virus (HCV) infection through the efficient recruitment and activation of inflammatory cells to infected liver. The CC-chemokine receptor (CCR) 5 is expressed on the several cells of the immune system and has been reported that the CCR5-59353T/C polymorphism may be associated with HCV infection. Hence, this study was aimed to determine the association of this polymorphism with susceptibility to HCV infection in Iranian patients. Material and methods: In this study, 100 HCV infected patients and 100 healthy individuals were randomly selected. Genomic DNA was extracted from blood buffy coat using the salting out method. The CCR5-59353(T/C) genotypes were determined using Allele Specific PCR method. The PCR products were electrophoresed on 1.5% agarose gel. The data were analyzed using Chi-square test. Results: The frequency of C variant allele was 42% in HCV infected patients and 37% in healthy individuals which was not statistically significant (P= 0.3). Also, the frequency of CCR5-59353C/C genotype was 16% in patients infected with HCV and 12% in healthy individuals and no significant difference was observed between the two groups (P= 0.4). Conclusion: Based on our findings, CCR5-593535T/C polymorphism is not effective in the study population as a host genetic factor in determining susceptibility to HCV infection.
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Type of Study: Research(Original) | Subject: genetic

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