alidadi H, Shokrzadeh M, Ahangar N. Frequency Evaluation of GSTM1 and GSTT1 Null Genotypes in a Healthy Population in Mazandaran Province. J Mazandaran Univ Med Sci 2014; 24 (119) :174-182
URL:
http://jmums.mazums.ac.ir/article-1-4796-en.html
Abstract: (7167 Views)
Background and purpose: Glutathion S-transferase (GSTs) are a family of detoxification phase
II enzymes that catalyze the conjugation of carcinogenic and cytotoxic componds with glutathion. GSTs
are polymorphic and deletion of GSTM1 and GSTT1 polymorphism is caused by lack of enzyme
production that lead to loss of antioxidant activity. In this study, we examined the frequency of GSTM1
and GSTT1 polymorphisms in a healthy population in Mazandaran province.
Materials and methods: In this experimental study, 250 non-relative healthy subjects in
Mazandaran province enrolled. They were residing in Sari and attended Sari Blood Transfusion Center
for blood donation. A total of 5 ml peripheral blood was taken from individuals. Genomic DNA was
extracted using DNGTM Plus Blood DNA kit. The Multiplex- PCR method was used for the detection of
genotypes in each subject.
Results: The subjects consisted of 210 men (84%) and 40 women (16%).The frequencies of the
GSTM1 and GSTT1-null genotypes were 54 and 30%, respectively. For GSTM1 null genotype, the
frequencies in women and men were 60% and 52.85%, respectively. While the frequencies of GSTT1
null genotype in women and men were 42.5% and 27.6%, respectively. In both regions, the frequency of
null genotype was significantly higher in women (p<0.05).
Conclusion: The genotype frequencies of null GSTM1 and GSTT1 were found to be relatively
high in Mazandaran province and this could possibly have a role in increasing the susceptibility to some
cancers.