Volume 26, Issue 143 (12-2016)                   J Mazandaran Univ Med Sci 2016, 26(143): 20-27 | Back to browse issues page

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Avakh Majelan P, Mahdavi M, Pourmand M R, Yazdi M H, Khoramabadi N. Passive Immunity by Recombinant Anti-Methicillin-resistant Staphylococcus aureus ScaF Antibody in Mouse Model. J Mazandaran Univ Med Sci. 2016; 26 (143) :20-27
URL: http://jmums.mazums.ac.ir/article-1-8998-en.html
Abstract:   (3881 Views)

Background and purpose: Methicillin resistant Staphylococcus aureus (MRSA) is a community-associated pathogen that is so common in hospitals. Antibiotic resistance and poor clinical outcome provide great reasons for using immunization strategies based on antibodies. The aim of this study was to investigate passive immunity using recombinant anti-MRSA ScaF antibody in a mouse model.

Materials and methods: In this study, the recombinant protein, ScaF was purified through denaturation method. This protein combined with Freund’s adjuvant was injected into a rabbit to produce antibody. Then, the antibody produced against ScaF was injected to mice infected by S. aureus by two regimens of pre- and after infection treatment. Afterwards, the mortality rate in mice was compared between the control treatment group (vancomycin therapy) and negative control group (non-immune rabbit serum).

Results: Out of 10 mice in two groups of pre- and after infection treatment that received antibody against ScaF antigen 20% and 10% were protected, respectively, that did not show a significant difference compared with non-immune rabbit serum group (negative control).  

Conclusion: Passive immunization by antibody cannot play a considerable role in the control of infections caused by S. aureus. Therefore, other mechanisms of immune system for the protection against this bacterium including stimulating of cytokine-dependent cell-mediated immunity may be useful in prevention of infections caused by S. aureus.

Full-Text [PDF 548 kb]   (1150 Downloads)    
Type of Study: Research(Original) |

Add your comments about this article : Your username or Email:

Send email to the article author

© 2018 All Rights Reserved | Journal of Mazandaran University of Medical Sciences

Designed & Developed by : Yektaweb