Background and purpose: Mycotoxins are a group of toxic compounds produced by several species of fungi and aflatoxins are the most important toxins. M1 and M2 which are derivatives of aflatoxin B1 and B2 are resistant against thermal changes, such as pasteurization, sterilization and autoclave and their concentration will not decrease. They are transmitted to humans by milk and milk products and threaten the health of human and society. The aim of this study was to determine aflatoxin M1 in buffaloes milk in Ahvaz and Karoon, Iran using ELIZA method.

Materials and methods: In a cross-sectional study, 60 milk samples were randomly collected from two farms with large number of buffalos, in autumn. ELISA method was used to evaluate of aflatoxins the samples.

Results: The average level of aflatoxin M1 in two selected locations was (155.91 ng/l) which was more than standard level in Iran and the Codex Alimentarius.

Conclusion: According to this research, there was a strong relationship between presence of toxin in milk with the animals’ diet and method of storing their foods.Background and purpose: Mycotoxins are a group of toxic compounds produced by several species of fungi and aflatoxins are the most important toxins. M1 and M2 which are derivatives of aflatoxin B1 and B2 are resistant against thermal changes, such as pasteurization, sterilization and autoclave and their concentration will not decrease. They are transmitted to humans by milk and milk products and threaten the health of human and society. The aim of this study was to determine aflatoxin M1 in buffaloes milk in Ahvaz and Karoon, Iran using ELIZA method.

Materials and methods: In a cross-sectional study, 60 milk samples were randomly collected from two farms with large number of buffalos, in autumn. ELISA method was used to evaluate of aflatoxins the samples.

Results: The average level of aflatoxin M1 in two selected locations was (155.91 ng/l) which was more than standard level in Iran and the Codex Alimentarius.

Conclusion: According to this research, there was a strong relationship between presence of toxin in milk with the animals’ diet and method of storing their foods.

Background and purpose: Mycotoxins are a group of toxic compounds produced by several species of fungi and aflatoxins are the most important toxins. M1 and M2 which are derivatives of aflatoxin B1 and B2 are resistant against thermal changes, such as pasteurization, sterilization and autoclave and their concentration will not decrease. They are transmitted to humans by milk and milk products and threaten the health of human and society. The aim of this study was to determine aflatoxin M1 in buffaloes milk in Ahvaz and Karoon, Iran using ELIZA method.

Materials and methods: In a cross-sectional study, 60 milk samples were randomly collected from two farms with large number of buffalos, in autumn. ELISA method was used to evaluate of aflatoxins the samples.

Results: The average level of aflatoxin M1 in two selected locations was (155.91 ng/l) which was more than standard level in Iran and the Codex Alimentarius.

Conclusion: According to this research, there was a strong relationship between presence of toxin in milk with the animals’ diet and method of storing their foods.Background and purpose: Mycotoxins are a group of toxic compounds produced by several species of fungi and aflatoxins are the most important toxins. M1 and M2 which are derivatives of aflatoxin B1 and B2 are resistant against thermal changes, such as pasteurization, sterilization and autoclave and their concentration will not decrease. They are transmitted to humans by milk and milk products and threaten the health of human and society. The aim of this study was to determine aflatoxin M1 in buffaloes milk in Ahvaz and Karoon, Iran using ELIZA method.

Materials and methods: In a cross-sectional study, 60 milk samples were randomly collected from two farms with large number of buffalos, in autumn. ELISA method was used to evaluate of aflatoxins the samples.

Results: The average level of aflatoxin M1 in two selected locations was (155.91 ng/l) which was more than standard level in Iran and the Codex Alimentarius.

Conclusion: According to this research, there was a strong relationship between presence of toxin in milk with the animals’ diet and method of storing their foods.

Background and purpose: Mycotoxins are a group of toxic compounds produced by several species of fungi and aflatoxins are the most important toxins. M1 and M2 which are derivatives of aflatoxin B1 and B2 are resistant against thermal changes, such as pasteurization, sterilization and autoclave and their concentration will not decrease. They are transmitted to humans by milk and milk products and threaten the health of human and society. The aim of this study was to determine aflatoxin M1 in buffaloes milk in Ahvaz and Karoon, Iran using ELIZA method.

Materials and methods: In a cross-sectional study, 60 milk samples were randomly collected from two farms with large number of buffalos, in autumn. ELISA method was used to evaluate of aflatoxins the samples.

Results: The average level of aflatoxin M1 in two selected locations was (155.91 ng/l) which was more than standard level in Iran and the Codex Alimentarius.

Conclusion: According to this research, there was a strong relationship between presence of toxin in milk with the animals’ diet and method of storing their foods.Background and purpose: Mycotoxins are a group of toxic compounds produced by several species of fungi and aflatoxins are the most important toxins. M1 and M2 which are derivatives of aflatoxin B1 and B2 are resistant against thermal changes, such as pasteurization, sterilization and autoclave and their concentration will not decrease. They are transmitted to humans by milk and milk products and threaten the health of human and society. The aim of this study was to determine aflatoxin M1 in buffaloes milk in Ahvaz and Karoon, Iran using ELIZA method.

Materials and methods: In a cross-sectional study, 60 milk samples were randomly collected from two farms with large number of buffalos, in autumn. ELISA method was used to evaluate of aflatoxins the samples.

Results: The average level of aflatoxin M1 in two selected locations was (155.91 ng/l) which was more than standard level in Iran and the Codex Alimentarius.

Conclusion: According to this research, there was a strong relationship between presence of toxin in milk with the animals’ diet and method of storing their foods.

Background and purpose: Mycotoxins are a group of toxic compounds produced by several species of fungi and aflatoxins are the most important toxins. M1 and M2 which are derivatives of aflatoxin B1 and B2 are resistant against thermal changes, such as pasteurization, sterilization and autoclave and their concentration will not decrease. They are transmitted to humans by milk and milk products and threaten the health of human and society. The aim of this study was to determine aflatoxin M1 in buffaloes milk in Ahvaz and Karoon, Iran using ELIZA method.

Materials and methods: In a cross-sectional study, 60 milk samples were randomly collected from two farms with large number of buffalos, in autumn. ELISA method was used to evaluate of aflatoxins the samples.

Results: The average level of aflatoxin M1 in two selected locations was (155.91 ng/l) which was more than standard level in Iran and the Codex Alimentarius.

Conclusion: According to this research, there was a strong relationship between presence of toxin in milk with the animals’ diet and method of storing their foods.Background and purpose: Mycotoxins are a group of toxic compounds produced by several species of fungi and aflatoxins are the most important toxins. M1 and M2 which are derivatives of aflatoxin B1 and B2 are resistant against thermal changes, such as pasteurization, sterilization and autoclave and their concentration will not decrease. They are transmitted to humans by milk and milk products and threaten the health of human and society. The aim of this study was to determine aflatoxin M1 in buffaloes milk in Ahvaz and Karoon, Iran using ELIZA method.

Materials and methods: In a cross-sectional study, 60 milk samples were randomly collected from two farms with large number of buffalos, in autumn. ELISA method was used to evaluate of aflatoxins the samples.

Results: The average level of aflatoxin M1 in two selected locations was (155.91 ng/l) which was more than standard level in Iran and the Codex Alimentarius.

Conclusion: According to this research, there was a strong relationship between presence of toxin in milk with the animals’ diet and method of storing their foods.

Background and purpose: Mycotoxins are a group of toxic compounds produced by several species of fungi and aflatoxins are the most important toxins. M1 and M2 which are derivatives of aflatoxin B1 and B2 are resistant against thermal changes, such as pasteurization, sterilization and autoclave and their concentration will not decrease. They are transmitted to humans by milk and milk products and threaten the health of human and society. The aim of this study was to determine aflatoxin M1 in buffaloes milk in Ahvaz and Karoon, Iran using ELIZA method.

Materials and methods: In a cross-sectional study, 60 milk samples were randomly collected from two farms with large number of buffalos, in autumn. ELISA method was used to evaluate of aflatoxins the samples.

Results: The average level of aflatoxin M1 in two selected locations was (155.91 ng/l) which was more than standard level in Iran and the Codex Alimentarius.

Conclusion: According to this research, there was a strong relationship between presence of toxin in milk with the animals’ diet and method of storing their foods.

Background and purpose: Mycotoxins are a group of toxic compounds produced by several species of fungi and aflatoxins are the most important toxins. M1 and M2 which are derivatives of aflatoxin B1 and B2 are resistant against thermal changes, such as pasteurization, sterilization and autoclave and their concentration will not decrease. They are transmitted to humans by milk and milk products and threaten the health of human and society. The aim of this study was to determine aflatoxin M1 in buffaloes milk in Ahvaz and Karoon, Iran using ELIZA method.

Materials and methods: In a cross-sectional study, 60 milk samples were randomly collected from two farms with large number of buffalos, in autumn. ELISA method was used to evaluate of aflatoxins the samples.

Results: The average level of aflatoxin M1 in two selected locations was (155.91 ng/l) which was more than standard level in Iran and the Codex Alimentarius.

Conclusion: According to this research, there was a strong relationship between presence of toxin in milk with the animals’ diet and method of storing their foods.